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Synthesis of PLGA–Lipid Hybrid Nanoparticles for siRNA Delivery Using the Emulsion Method PLGA-PEG–Lipid Nanoparticles for siRNA Delivery

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RNA Nanostructures

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1632))

Abstract

The effective delivery of small interfering RNA (siRNA) to tumor cells remains a challenge for applications in cancer therapy. The development of polymeric nanoparticles with high siRNA loading efficacy has shown great potential for cancer targets. Double emulsion solvent evaporation technique is a useful tool for encapsulation of hydrophilic molecules (e.g., siRNA). Here we describe a versatile platform for siRNA delivery based on PLGA-PEG–cationic lipid nanoparticles by using the double emulsion method. The resulting nanoparticles show high encapsulation efficiency for siRNA (up to 90%) and demonstrate effective downregulation of the target genes in vitro and vivo.

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Author information

Authors and Affiliations

  1. Otto H. York Department of Chemical, Biological and Pharmaceutical Engineering, New Jersey Institute of Technology, University Heights, Newark, NJ, 07102, USA

    Lei Wang, Benjamin Griffel & Xiaoyang Xu

  2. College of Chemistry, Chemical Engineering and Materials Science, Soochow University, 199 Ren-Ai Road, Suzhou Industrial Park, Suzhou, Jiangsu, 215123, China

    Lei Wang

  3. Bard High School Early College, Newark, NJ, 07103, USA

    Benjamin Griffel

Authors
  1. Lei Wang
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  2. Benjamin Griffel
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  3. Xiaoyang Xu
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Corresponding author

Correspondence to Xiaoyang Xu .

Editor information

Editors and Affiliations

  1. Frederick National Laboratory for Cancer Research, Leidos Biomedical Research, Frederick, Maryland, USA

    Eckart Bindewald

  2. RNA Structure and Design Section, RNA Biology Laboratory, National Cancer Institute, National Institutes of Health, Frederick, Maryland, USA

    Bruce A. Shapiro

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Wang, L., Griffel, B., Xu, X. (2017). Synthesis of PLGA–Lipid Hybrid Nanoparticles for siRNA Delivery Using the Emulsion Method PLGA-PEG–Lipid Nanoparticles for siRNA Delivery. In: Bindewald, E., Shapiro, B. (eds) RNA Nanostructures . Methods in Molecular Biology, vol 1632. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7138-1_15

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  • DOI: https://doi.org/10.1007/978-1-4939-7138-1_15

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7137-4

  • Online ISBN: 978-1-4939-7138-1

  • eBook Packages: Springer Protocols

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