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Detection of Free Polyamines in Plants Subjected to Abiotic Stresses by High-Performance Liquid Chromatography (HPLC)

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Plant Stress Tolerance

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1631))

Abstract

High-performance liquid chromatography (HPLC) is a sensitive, rapid, and accurate technique to detect and characterize various metabolites from plants. The metabolites are extracted with different solvents and eluted with appropriate mobile phases in a designed HPLC program. Polyamines are known to accumulate under abiotic stress conditions in various plant species and thought to provide protection against oxidative stress by scavenging reactive oxygen species. Here, we describe a common method to detect the free polyamines in plant tissues both qualitatively and quantitatively.

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Acknowledgment

This work was supported by China Postdoctoral Science Foundation (K3080215805) and Natural Science Foundation of China (31320103908).

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Authors and Affiliations

  1. College of Horticulture and Forestry Sciences, Huazhong Agricultural University, 430070, Wuhan, China

    Xiaoqing Gong & Ji-Hong Liu

Authors
  1. Xiaoqing Gong
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  2. Ji-Hong Liu
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Corresponding author

Correspondence to Ji-Hong Liu .

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Editors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, Oklahoma, USA

    Ramanjulu Sunkar

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Gong, X., Liu, JH. (2017). Detection of Free Polyamines in Plants Subjected to Abiotic Stresses by High-Performance Liquid Chromatography (HPLC). In: Sunkar, R. (eds) Plant Stress Tolerance. Methods in Molecular Biology, vol 1631. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7136-7_19

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  • DOI: https://doi.org/10.1007/978-1-4939-7136-7_19

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7134-3

  • Online ISBN: 978-1-4939-7136-7

  • eBook Packages: Springer Protocols

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