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Profiling Abscisic Acid-Induced Changes in Fatty Acid Composition in Mosses

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Plant Stress Tolerance

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1631))

Abstract

In plants, change in lipid composition is a common response to various abiotic stresses. Lipid constituents of bryophytes are of particular interest as they differ from that of flowering plants. Unlike higher plants, mosses have high content of very long-chain polyunsaturated fatty acids. Such lipids are considered to be important for survival of nonvascular plants. Here, using abscisic acid (ABA )-induced changes in lipid composition in Physcomitrella patens as an example, a protocol for total lipid extraction and quantification by gas chromatography (GC) coupled with flame ionization detector (FID) is described.

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Acknowledgments

National Science Foundation Grant (NSF-IOS#1456917) supports AK’s research on moss.

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Authors and Affiliations

  1. Department of Biological Sciences, East Tennessee State University, Johnson City, TN, USA

    Suhas Shinde, Shivakumar Devaiah & Aruna Kilaru

Authors
  1. Suhas Shinde
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  2. Shivakumar Devaiah
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  3. Aruna Kilaru
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Corresponding author

Correspondence to Aruna Kilaru .

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Editors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, Oklahoma, USA

    Ramanjulu Sunkar

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Shinde, S., Devaiah, S., Kilaru, A. (2017). Profiling Abscisic Acid-Induced Changes in Fatty Acid Composition in Mosses. In: Sunkar, R. (eds) Plant Stress Tolerance. Methods in Molecular Biology, vol 1631. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7136-7_18

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  • DOI: https://doi.org/10.1007/978-1-4939-7136-7_18

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7134-3

  • Online ISBN: 978-1-4939-7136-7

  • eBook Packages: Springer Protocols

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