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Zymographic Method for Distinguishing Different Classes of Superoxide Dismutases in Plants

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Plant Stress Tolerance

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1631))

Abstract

In plants, especially in chloroplasts, superoxide radical is generated when an electron is transferred to dimolecular O2 due to decreased activity of Photosystem I. The superoxide (O2 −) radical accumulation is more rampant in plants exposed to abiotic stresses due to oxidation of photosystem components. Excessive superoxide radical accumulation will lead to oxidative damage to the cellular macromolecules. The ubiquitous superoxide dismutases (SODs) represent critical enzymatic antioxidant system present in cells, which can catalyze the disproportion of superoxide (O2 −) radical rapidly into hydrogen peroxide (H2O2) and molecular oxygen. Depending on the metal cofactor present, the plant SODs are classified into Cu/ZnSOD, MnSOD, and FeSOD. The activity of SODs can be quantified zymographically. Additionally, using this method, different classes of SODs can be distinguished by using H2O2, KCN, and NaN3.

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References

  1. Asada K, Takahashi M (1987). Production and scavenging of active oxygen in chloroplasts. In DJ Kyle, CB Osmond, CJ Arntzen, (eds), Photoinhibition Elsevier, Amsterdam, pp 227–287

    Google Scholar 

  2. Vázquez-Juárez R, Vargas-Albores F, Ochoa JL (1993) A computer program to calculate superoxide dismutase activity in crude extracts. J Microbiol Methods 17:239–244

    Google Scholar 

  3. Khutan H, Haussmann HJ, Werringloe J (1986) A spectrophotometric assay for superoxide dismutase activities in crude tissue fractions. Biochem J 237:175–180

    Article  Google Scholar 

  4. Culotta VC, Yang M, O'Halloran TV (2006) Activation of superoxide dismutases: putting the metal to the pedal. Biochim Biophys Acta 1763:747–758

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Chen CN, Pan SM (1996) Assay of superoxide dismutase activity by combining electrophoresis and densitometry. Bot Bull Acad Sin 37:107–111

    CAS  Google Scholar 

  6. Alscher RG, Erturk N, Heath LS (2002) Role of superoxide dismutases (SODs) in controlling oxidative stress in plants. J Exp Bot 53:1331–1341

    Article  CAS  PubMed  Google Scholar 

  7. Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 44:276–287

    Article  CAS  PubMed  Google Scholar 

  8. Lomate PR, Sangole KP, Sunkar R, Hivrale VK (2015) Superoxide dismutase activities in the midgut of Helicoverpaarmigera larvae: identification and biochemical properties of a manganese superoxide dismutase. Insect Physiology 5:13–20

    Google Scholar 

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Acknowledgment

ARJ gratefully acknowledges the BARTI, Pune for providing the junior research fellowship.

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Authors and Affiliations

  1. Department of Biochemistry, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad, MS, 431004, India

    Ashwini R. Jamdhade & Vandana K. Hivrale

  2. Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK, 74078, USA

    Ramanjulu Sunkar

Authors
  1. Ashwini R. Jamdhade
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  2. Ramanjulu Sunkar
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  3. Vandana K. Hivrale
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Corresponding author

Correspondence to Vandana K. Hivrale .

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Editors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, Oklahoma, USA

    Ramanjulu Sunkar

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Jamdhade, A.R., Sunkar, R., Hivrale, V.K. (2017). Zymographic Method for Distinguishing Different Classes of Superoxide Dismutases in Plants. In: Sunkar, R. (eds) Plant Stress Tolerance. Methods in Molecular Biology, vol 1631. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7136-7_13

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  • DOI: https://doi.org/10.1007/978-1-4939-7136-7_13

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7134-3

  • Online ISBN: 978-1-4939-7136-7

  • eBook Packages: Springer Protocols

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