Abstract
Protein phosphorylation is a key signaling mechanism during the plant biotic and abiotic stress response. Signaling cascades communicate between the cell surface, where the stress is perceived, and the nucleus, where a response can be enacted. Many of these signals involve the specific, transient phosphorylation of proteins by kinases, a signal which is usually amplified through cascades. The advent of high-throughput phosphoproteomics, pioneered mainly in yeast and mammalian cells, has made it possible to discover novel phosphorylation events rapidly and efficiently in a data-dependent manner and this has greatly enlarged our understanding of the plant’s response to stress. This chapter describes a simple gel-free protocol for high-throughput phosphoproteomics, which is amenable to most labs engaged in plant stress research.
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Acknowledgments
Funding for proteomics research to CR originates from Agriculture and Agrifood Canada. I thank Slavica Djuric-Ciganovic for critical comments on the manuscript.
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Rampitsch, C. (2017). Phosphoproteomics Analysis for Probing Plant Stress Tolerance. In: Sunkar, R. (eds) Plant Stress Tolerance. Methods in Molecular Biology, vol 1631. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7136-7_11
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DOI: https://doi.org/10.1007/978-1-4939-7136-7_11
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