Skip to main content
SpringerLink
Log in

Epigenetics and RNA Processing: Connections to Drought, Salt, and ABA?

  • Protocol
  • First Online:
Plant Stress Tolerance

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1631))

Abstract

There have been great research advances in epigenetics, RNA splicing, and mRNA processing over recent years. In parallel, there have been many advances in abiotic stress and Abscisic Acid (ABA) signaling. Here we overview studies that have examined stress-induced changes in the epigenome and RNA processing as well as cases where disrupting these processes changes the plant response to abiotic stress. We also highlight some examples where specific connections of stress or ABA signaling to epigenetics or RNA processing have been found. By implication, this also points out cases where such mechanistic connections are likely to exist but are yet to be characterized. In the absence of such specific connections to stress signaling, it should be kept in mind that stress sensitivity phenotypes of some epigenetic or RNA processing mutants maybe the result of indirect, pleiotropic effects and thus may perhaps not indicate a direct function in stress acclimation.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 129.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 179.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Feng W, Lindner H, Robbins NE, Dinneny JR (2016) Growing out of stress: the role of cell-and organ-scale growth control in plant water-stress responses. Plant Cell 28:1769–1782

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Skirycz A, Inze D (2010) More from less: plant growth under limited water. Curr Opin Biotechnol 21:197–203

    Article  CAS  PubMed  Google Scholar 

  3. Verslues PE, Agarwal M, Katiyar-Agarwal S, Zhu J, Zhu JK (2006) Methods and concepts in quantifying resistance to drought, salt and freezing, abiotic stresses that affect plant water status. Plant J 45:523–539

    Article  CAS  PubMed  Google Scholar 

  4. Haswell ES, Verslues PE (2015) The ongoing search for the molecular basis of plant osmosensing. J Gen Physiol 145:389–394

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Cutler SR, Rodriguez PL, Finkelstein RR, Abrams SR (2010) Abscisic acid: emergence of a core signaling network. Ann Rev Plant Biol 61:651–679

    Article  CAS  Google Scholar 

  6. Raghavendra AS, Gonugunta VK, Christmann A, Grill E (2010) ABA perception and signalling. Trends Plant Sci 15:395–401

    Article  CAS  PubMed  Google Scholar 

  7. Verslues PE (2016) ABA and cytokinins: challenge and opportunity for plant stress research. Plant Mol Biol 91:629–640

    Article  CAS  PubMed  Google Scholar 

  8. Nakashima K, Yamaguchi-Shinozaki K, Shinozaki K (2014) The transcriptional regulatory network in the drought response and its crosstalk in abiotic stress responses including drought, cold, and heat. In: Jain M, Garg R, Varshney RK (eds) Abiotic stress: molecular genetics and genomics. Frontiers E-Books, Lausanne, pp 25–31

    Google Scholar 

  9. Yoshida T, Mogami J, Yamaguchi-Shinozaki K (2014) ABA-dependent and ABA-independent signaling in response to osmotic stress in plants. Curr Opin Plant Biol 21:133–139

    Article  CAS  PubMed  Google Scholar 

  10. Stockinger EJ, Gilmour SJ, Thomashow MF (1997) Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator that binds to the C-repeat/DRE, a cis-acting DNA regulatory element that stimulates transcription in response to low temperature and water deficit. Proc Natl Acad Sci U S A 94:1035–1040

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Shinozaki K, Yamaguchi-Shinozaki K (2000) Molecular responses to dehydration and low temperature: differences and cross-talk between two stress signaling pathways. Curr Opin Plant Biol 3:217–223

    Article  CAS  PubMed  Google Scholar 

  12. Sharp RE, LeNoble ME (2002) ABA, ethylene and the control of shoot and root growth under water stress. J Exp Bot 53:33–37

    Article  CAS  PubMed  Google Scholar 

  13. Crisp PA, Ganguly D, Eichten SR, Borevitz JO, Pogson BJ (2016) Reconsidering plant memory: Intersections between stress recovery, RNA turnover, and epigenetics. Sci Adv 2:e1501340

    Article  PubMed  PubMed Central  Google Scholar 

  14. Virlouvet L, Ding Y, Fujii H, Avramova Z, Fromm M (2014) ABA signaling is necessary but not sufficient for RD29B transcriptional memory during successive dehydration stresses in Arabidopsis thaliana. Plant J 79:150–161

    Article  CAS  PubMed  Google Scholar 

  15. Ding Y, Fromm M, Avramova Z (2012) Multiple exposures to drought 'train' transcriptional responses in Arabidopsis. Nat Commun 3:740

    Article  PubMed  Google Scholar 

  16. Shen Y, Issakidis-Bourguet E, Zhou D-X (2016) Perspectives on the interactions between metabolism, redox, and epigenetics in plants. J Exp Bot 67:5291–5300

    Article  CAS  PubMed  Google Scholar 

  17. Van Oosten MJ, Bressan RA, Zhu J-K, Bohnert HJ, Chinnusamy V (2014) The role of the epigenome in gene expression control and the epimark changes in response to the environment. Crit Rev Plant Sci 33:64–87

    Article  Google Scholar 

  18. Kinoshita T, Seki M (2014) Epigenetic memory for stress response and adaptation in plants. Plant Cell Physiol 55:1859–1863

    Article  CAS  PubMed  Google Scholar 

  19. Liu X, Luo M, Yang S, Wu K (2015) Role of epigenetic modifications in plant responses to environmental stresses. In: Pontes O, Jin H (eds) Nuclear functions in plant transcription, signaling and development. New York, NY, Springer New York, pp 81–92

    Google Scholar 

  20. Colaneri AC, Jones AM (2013) Genome-wide quantitative identification of DNA differentially methylated sites in Arabidopsis seedlings growing at different water potential. PLoS One 8:e59878

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Zong W, Zhong X, You J, Xiong L (2013) Genome-wide profiling of histone H3K4-tri-methylation and gene expression in rice under drought stress. Plant Mol Biol 81:175–188

    Article  CAS  PubMed  Google Scholar 

  22. Wibowo A, Becker C, Marconi G, Durr J, Price J, Hagmann J, Papareddy R, Putra H, Kageyama J, Becker J et al (2016) Hyperosmotic stress memory in Arabidopsis is mediated by distinct epigenetically labile sites in the genome and is restricted in the male germline by DNA glycosylase activity. eLife 5:e13546

    Article  PubMed  PubMed Central  Google Scholar 

  23. Chwialkowska K, Nowakowska U, Mroziewicz A, Szarejko I, Kwasniewski M (2016) Water-deficiency conditions differently modulate the methylome of roots and leaves in barley (Hordeum vulgare L.) J Exp Bot 67:1109–1121

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Sani E, Herzyk P, Perrella G, Colot V, Amtmann A (2013) Hyperosmotic priming of Arabidopsis seedlings establishes a long-term somatic memory accompanied by specific changes of the epigenome. Genome Biol 14:R59

    Article  PubMed  PubMed Central  Google Scholar 

  25. Filichkin S, Priest HD, Megraw M, Mockler TC (2015) Alternative splicing in plants: directing traffic at the crossroads of adaptation and environmental stress. Curr Opin Plant Biol 24:125–135

    Article  CAS  PubMed  Google Scholar 

  26. Kuhn JM, Schroeder JI (2003) Impacts of altered RNA metabolism on abscisic acid signaling. Curr Opin Plant Biol 6:463–469

    Article  CAS  PubMed  Google Scholar 

  27. Saez A, Rodrigues A, Santiago J, Rubio S, Rodriguez PL (2008) HAB1–SWI3B interaction reveals a link between abscisic acid signaling and putative SWI/SNF chromatin-remodeling complexes in Arabidopsis. Plant Cell 20:2972–2988

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  28. Hurtado L, Farrona S, Reyes JC (2006) The putative SWI/SNF complex subunit BRAHMA activates flower homeotic genes in Arabidopsis thaliana. Plant Mol Biol 62:291–304

    Article  CAS  PubMed  Google Scholar 

  29. Wagner D, Meyerowitz EM (2002) SPLAYED, a novel SWI/SNF ATPase homolog, controls reproductive development in Arabidopsis. Curr Biol 12:85–94

    Article  CAS  PubMed  Google Scholar 

  30. Brzeski J, Podstolski W, Olczak K, Jerzmanowski A (1999) Identification and analysis of the Arabidopsis thaliana BSH gene, a member of the SNF5 gene family. Nucleic Acids Res 27:2393–2399

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  31. Sarnowska E, Gratkowska DM, Sacharowski SP, Cwiek P, Tohge T, Fernie AR, Siedlecki JA, Koncz C, Sarnowski TJ (2016) The role of SWI/SNF chromatin remodeling complexes in hormone crosstalk. Trends Plant Sci 21:594–608

    Article  CAS  PubMed  Google Scholar 

  32. Sarnowski TJ, Ríos G, Jásik J, Świeżewski S, Kaczanowski S, Li Y, Kwiatkowska A, Pawlikowska K, Koźbiał M, Koźbiał P (2005) SWI3 subunits of putative SWI/SNF chromatin-remodeling complexes play distinct roles during Arabidopsis development. Plant Cell 17:2454–2472

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  33. Han S-K, Sang Y, Rodrigues A, Wu M-F, Rodriguez PL, Wagner D (2012) The SWI2/SNF2 chromatin remodeling ATPase BRAHMA represses abscisic acid responses in the absence of the stress stimulus in Arabidopsis. Plant Cell 24:4892–4906

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  34. Peirats-Llobet M, Han SK, Gonzalez-Guzman M, Jeong CW, Rodriguez L, Belda-Palazon B, Wagner D, Rodriguez PL (2016) A direct link between abscisic acid sensing and the chromatin-remodeling ATPase BRAHMA via core ABA signaling pathway components. Mol Plant 9:136–147

    Article  CAS  PubMed  Google Scholar 

  35. Perruc E, Kinoshita N, Lopez-Molina L (2007) The role of chromatin-remodeling factor PKL in balancing osmotic stress responses during Arabidopsis seed germination. Plant J 52:927–936

    Article  CAS  PubMed  Google Scholar 

  36. Yuan L, Liu X, Luo M, Yang S, Wu K (2013) Involvement of histone modifications in plant abiotic stress responses. J Integr Plant Biol 55:892–901

    Google Scholar 

  37. Kim JM, Sasaki T, Ueda M, Sako K, Seki M (2015) Chromatin changes in response to drought, salinity, heat, and cold stresses in plants. Front Plant Sci 6:114

    PubMed  PubMed Central  Google Scholar 

  38. Han SK, Wagner D (2014) Role of chromatin in water stress responses in plants. J Exp Bot 65:2785–2799

    Article  CAS  PubMed  Google Scholar 

  39. Probst AV, Mittelsten Scheid O (2015) Stress-induced structural changes in plant chromatin. Curr Opin Plant Biol 27:8–16

    Article  CAS  PubMed  Google Scholar 

  40. Shahbazian MD, Grunstein M (2007) Functions of site-specific histone acetylation and deacetylation. Annu Rev Biochem 76:75–100

    Article  CAS  PubMed  Google Scholar 

  41. Lee KK, Workman JL (2007) Histone acetyltransferase complexes: one size doesn't fit all. Nat Rev Mol Cell Biol 8:284–295

    Article  CAS  PubMed  Google Scholar 

  42. Baker S, Grant P (2007) The SAGA continues: expanding the cellular role of a transcriptional co-activator complex. Oncogene 26:5329–5340

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  43. Hark AT, Vlachonasios KE, Pavangadkar KA, Rao S, Gordon H, Adamakis ID, Kaldis A, Thomashow MF, Triezenberg SJ (2009) Two Arabidopsis orthologs of the transcriptional coactivator ADA2 have distinct biological functions. Biochim Biophys Acta 1789:117–124

    Article  CAS  PubMed  Google Scholar 

  44. Kaldis A, Tsementzi D, Tanriverdi O, Vlachonasios KE (2011) Arabidopsis thaliana transcriptional co-activators ADA2b and SGF29a are implicated in salt stress responses. Planta 233:749–762

    Article  CAS  PubMed  Google Scholar 

  45. Mao YP, Pavangadkar KA, Thomashow MF, Triezenberg SJ (2006) Physical and functional interactions of Arabidopsis ADA2 transcriptional coactivator proteins with the acetyltransferase GCN5 and with the cold-induced transcription factor CBF1. Biochim Biophys Acta 1759:69–79

    Article  CAS  PubMed  Google Scholar 

  46. Stockinger EJ, Mao Y, Regier MK, Triezenberg SJ, Thomashow MF (2001) Transcriptional adaptor and histone acetyltransferase proteins in Arabidopsis and their interactions with CBF1, a transcriptional activator involved in cold-regulated gene expression. Nucleic Acid Res 29:1524–1533

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  47. Chen Z, Zhang H, Jablonowski D, Zhou X, Ren X, Hong X, Schaffrath R, Zhu JK, Gong Z (2006) Mutations in ABO1/ELO2, a subunit of holo-Elongator, increase abscisic acid sensitivity and drought tolerance in Arabidopsis thaliana. Mol Cell Biol 26:6902–6912

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  48. Versees W, De Groeve S, Van Lijsebettens M (2010) Elongator, a conserved multitasking complex? Mol Microbiol 76:1065–1069

    Article  CAS  PubMed  Google Scholar 

  49. Sridha S, Wu K (2006) Identification of AtHD2C as a novel regulator of abscisic acid responses in Arabidopsis. Plant J 46:124–133

    Article  CAS  PubMed  Google Scholar 

  50. Chen LT, Luo M, Wang YY, Wu K (2010) Involvement of Arabidopsis histone deacetylase HDA6 in ABA and salt stress response. J Exp Bot 61:3345–3353

    Google Scholar 

  51. Chen LT, Wu K (2010) Role of histone deacetylases HDA6 and HDA19 in ABA and abiotic stress response. Plant Signal Behav 5:1318–1320

    Google Scholar 

  52. Luo M, Wang YY, Liu X, Yang S, Lu Q, Cui Y, Wu K (2012) HD2C interacts with HDA6 and is involved in ABA and salt stress response in Arabidopsis. J Exp Bot 63:3297–3306

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  53. Zheng Y, Ding Y, Sun X, Xie S, Wang D, Liu X, Su L, Wei W, Pan L, Zhou D-X (2016) Histone deacetylase HDA9 negatively regulates salt and drought stress responsiveness in Arabidopsis. J Exp Bot 67:1703–1713

    Article  CAS  PubMed  Google Scholar 

  54. He X-J, Chen T, Zhu J-K (2011) Regulation and function of DNA methylation in plants and animals. Cell Res 21:442–465

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  55. Stroud H, Greenberg MV, Feng S, Bernatavichute YV, Jacobsen SE (2013) Comprehensive analysis of silencing mutants reveals complex regulation of the Arabidopsis methylome. Cell 152:352–364

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  56. Law JA, Jacobsen SE (2010) Establishing, maintaining and modifying DNA methylation patterns in plants and animals. Nat Rev Genet 11:204–220

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  57. Henderson IR, Jacobsen SE (2007) Epigenetic inheritance in plants. Nature 447:418–424

    Article  CAS  PubMed  Google Scholar 

  58. Matzke MA, Mosher RA (2014) RNA-directed DNA methylation: an epigenetic pathway of increasing complexity. Nat Rev Genet 15:394–408

    Article  CAS  PubMed  Google Scholar 

  59. Zhang H, Zhu J-K (2012) Seeing the forest for the trees: a wide perspective on RNA-directed DNA methylation. Genes Dev 26:1769–1773

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  60. Kovarik A, Koukalova B, Bezde M, Opatrn Z (1997) Hypermethylation of tobacco heterochromatic loci in response to osmotic stress. Theor Appl Genet 95:301–306

    Article  Google Scholar 

  61. Labra M, Ghiani A, Citterio S, Sgorbati S, Sala F, Vannini C, Ruffini-Castiglione M, Bracale M (2002) Analysis of cytosine methylation pattern in response to water deficit in pea root tips. Plant Biol 4:694–699

    Article  CAS  Google Scholar 

  62. Suji K, Joel AJ (2010) An epigenetic change in rice cultivars under water stress conditions. Electron J Plant Breed 1:1142–1143

    Google Scholar 

  63. Tan MP (2010) Analysis of DNA methylation of maize in response to osmotic and salt stress based on methylation-sensitive amplified polymorphism. Plant Physiol Biochem 48:21–26

    Article  CAS  PubMed  Google Scholar 

  64. Garg R, Chevala VN, Shankar R, Jain M (2015) Divergent DNA methylation patterns associated with gene expression in rice cultivars with contrasting drought and salinity stress response. Sci Rep 5:14992

    Article  Google Scholar 

  65. Kawakatsu T, Huang SSC, Jupe F, Sasaki E, Schmitz RJ, Urich MA, Castanon R, Nery JR, Barragan C, He YP et al (2016) Epigenomic diversity in a global collection of Arabidopsis thaliana accessions. Cell 166:492–505

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  66. Kawakatsu T, Stuart T, Valdes M, Breakfield N, Schmitz RJ, Nery JR, Urich MA, Han XW, Lister R, Benfey PN et al (2016) Unique cell-type-specific patterns of DNA methylation in the root meristem. Nat Plant 2:16058

    Article  CAS  Google Scholar 

  67. Song Y, Ji D, Li S, Wang P, Li Q, Xiang F (2012) The dynamic changes of DNA methylation and histone modifications of salt responsive transcription factor genes in soybean. PLoS One 7:e41274

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  68. Bilichak A, Ilnystkyy Y, Hollunder J, Kovalchuk I (2012) The progeny of Arabidopsis thaliana plants exposed to salt exhibit changes in DNA methylation, histone modifications and gene expression. PLoS One 7:e30515

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  69. Soppe WJ, Jasencakova Z, Houben A, Kakutani T, Meister A, Huang MS, Jacobsen SE, Schubert I, Fransz PF (2002) DNA methylation controls histone H3 lysine 9 methylation and heterochromatin assembly in Arabidopsis. EMBO J 21:6549–6559

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  70. Tariq M, Saze H, Probst AV, Lichota J, Habu Y, Paszkowski J (2003) Erasure of CpG methylation in Arabidopsis alters patterns of histone H3 methylation in heterochromatin. Proc Natl Acad Sci U S A 100:8823–8827

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  71. Duque P (2011) A role for SR proteins in plant stress responses. Plant Signal Behav 6:49–54

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  72. Filichkin SA, Priest HD, Givan SA, Shen R, Bryant DW, Fox SE, Wong WK, Mockler TC (2010) Genome-wide mapping of alternative splicing in Arabidopsis thaliana. Genome Res 20:45–58

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  73. Thatcher SR, Danilevskaya ON, Meng X, Beatty M, Zastrow-Hayes G, Harris C, Van Allen B, Habben J, Li B (2016) Genome-wide analysis of alternative splicing during development and drought stress in maize. Plant Physiol 170:586–599

    Article  CAS  PubMed  Google Scholar 

  74. Mazzucotelli E, Mastrangelo AM, Crosatti C, Guerra D, Stanca AM, Cattivelli L (2008) Abiotic stress response in plants: when post-transcriptional and post-translational regulations control transcription. Plant Sci 174:420–431

    Article  CAS  Google Scholar 

  75. Raczynska KD, Stepien A, Kierzkowski D, Kalak M, Bajczyk M, McNicol J, Simpson CG, Szweykowska-Kulinska Z, Brown JWS, Jarmolowski A (2014) The SERRATE protein is involved in alternative splicing in Arabidopsis thaliana. Nucleic Acid Res 42:1224–1244

    Article  CAS  PubMed  Google Scholar 

  76. Remy E, Cabrito TR, Batista RA, Hussein MA, Teixeira MC, Athanasiadis A, Sa-Correia I, Duque P (2014) Intron retention in the 5'UTR of the novel ZIF2 transporter enhances translation to promote zinc tolerance in Arabidopsis. PLoS Genet 10:e1004375

    Article  PubMed  PubMed Central  Google Scholar 

  77. Chang CY, Lin WD, Tu SL (2014) Genome-wide analysis of heat-sensitive alternative splicing in Physcomitrella patens. Plant Physiol 165:826–840

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  78. Wu HP, Su YS, Chen HC, Chen YR, Wu CC, Lin WD, Tu SL (2014) Genome-wide analysis of light-regulated alternative splicing mediated by photoreceptors in Physcomitrella patens. Genome Biol 15:R10

    Article  PubMed  PubMed Central  Google Scholar 

  79. Wang Z, Ji H, Yuan B, Wang S, Su C, Yao B, Zhao H, Li X (2015) ABA signalling is fine-tuned by antagonistic HAB1 variants. Nat Commun 6:8138

    Article  PubMed  Google Scholar 

  80. Zhan X, Qian B, Cao F, Wu W, Yang L, Guan Q, Gu X, Wang P, Okusolubo TA, Dunn SL et al (2015) An Arabidopsis PWI and RRM motif-containing protein is critical for pre-mRNA splicing and ABA responses. Nat Commun 6:8139

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  81. Sugliani M, Brambilla V, Clerkx EJ, Koornneef M, Soppe WJ (2010) The conserved splicing factor SUA controls alternative splicing of the developmental regulator ABI3 in Arabidopsis. Plant Cell 22:1936–1946

    Google Scholar 

  82. Umezawa T, Sugiyama N, Takahashi F, Anderson JC, Ishihama Y, Peck SC, Shinozaki K (2013) Genetics and phosphoproteomics reveal a protein phosphorylation network in the Abscisic Acid signaling pathway in Arabidopsis thaliana. Sci Signal 6:270

    Article  Google Scholar 

  83. Wang PC, Xue L, Batelli G, Lee S, Hou YJ, Van Oosten MJ, Zhang HM, Tao WA, Zhu JK (2013) Quantitative phosphoproteomics identifies SnRK2 protein kinase substrates and reveals the effectors of abscisic acid action. Proc Natl Acad Sci U S A 110:11205–11210

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  84. Ortega-Amaro MA, Rodriguez-Hernandez AA, Rodriguez-Kessler M, Hernandez-Lucero E, Rosales-Mendoza S, Ibanez-Salazar A, Delgado-Sanchez P, Jimenez-Bremont JF (2015) Overexpression of AtGRDP2, a novel glycine-rich domain protein, accelerates plant growth and improves stress tolerance. Front Plant Sci 5:782

    Article  PubMed  PubMed Central  Google Scholar 

  85. Rai AN, Tamirisa S, Rao KV, Kumar V, Suprasanna P (2016) Brassica RNA binding protein ERD4 is involved in conferring salt, drought tolerance and enhancing plant growth in Arabidopsis. Plant Mol Biol 90:375–387

    Article  CAS  PubMed  Google Scholar 

  86. Ambrosone A, Batelli G, Nurcato R, Aurilia V, Punzo P, Bangarusamy DK, Ruberti I, Sassi M, Leone A, Costa A et al (2015) The Arabidopsis RNA-binding protein AtRGGA regulates tolerance to salt and drought stress. Plant Physiol 168:292–306

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  87. Dhandapani G, Kanakachari M, Padmalatha KV, Phanindra MLV, Singh VK, Raghavendrarao S, Jayabalan N, Prabha AL, Kumar PA (2015) A gene encoding cold-circadian rhythm-RNA binding-like protein (CCR-Like) from upland Cotton (Gossypium hirsutum L.) confers tolerance to abiotic stresses in transgenic tobacco. Plant Mol Biol Rep 33:22–42

    Article  CAS  Google Scholar 

  88. Du J-L, Zhang S-W, Huang H-W, Cai T, Li L, Chen S, He X-J (2015) The splicing factor PRP31 is involved in transcriptional gene silencing and stress response in Arabidopsis. Mol Plant 8:1053–1068

    Article  CAS  PubMed  Google Scholar 

  89. Huh SU, Paek KH (2014) APUM5, encoding a Pumilio RNA binding protein, negatively regulates abiotic stress responsive gene expression. BMC Plant Biol 14:75

    Google Scholar 

  90. Cao S, Jiang L, Song S, Jing R, Xu G (2006) AtGRP7 is involved in the regulation of abscisic acid and stress responses in Arabidopsis. Cell Mol Biol Lett 11:526–535

    Article  CAS  PubMed  Google Scholar 

  91. Kim JY, Park SJ, Jang B, Jung CH, Ahn SJ, Goh CH, Cho K, Han O, Kang H (2007) Functional characterization of a glycine-rich RNA-binding protein 2 in Arabidopsis thaliana under abiotic stress conditions. Plant J 50:439–451

    Google Scholar 

  92. Kwak KJ, Kim YO, Kang H (2005) Characterization of transgenic Arabidopsis plants overexpressing GR-RBP4 under high salinity, dehydration, or cold stress. J Exp Bot 56:3007–3016

    Article  CAS  PubMed  Google Scholar 

  93. Xiong L, Gong Z, Rock CD, Subramanian S, Guo Y, Xu W, Galbraith D, Zhu JK (2001) Modulation of abscisic acid signal transduction and biosynthesis by an Sm-like protein in Arabidopsis. Dev Cell 1:771–781

    Article  CAS  PubMed  Google Scholar 

  94. Perea-Resa C, Hernandez-Verdeja T, Lopez-Cobollo R, del Mar Castellano M, Salinas J (2012) LSM proteins provide accurate splicing and decay of selected transcripts to ensure normal Arabidopsis development. Plant Cell 24:4930–4947

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  95. Golisz A, Sikorski PJ, Kruszka K, Kufel J (2013) Arabidopsis thaliana LSM proteins function in mRNA splicing and degradation. Nucleic Acid Res 41:6232–6249

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  96. Hugouvieux V, Kwak JM, Schroeder JI (2001) An mRNA cap binding protein, ABH1, modulates early abscisic acid signal transduction in Arabidopsis. Cell 106:477–487

    Article  CAS  PubMed  Google Scholar 

  97. Hugouvieux V, Murata Y, Young JJ, Kwak JM, Mackesy DZ, Schroeder JI (2002) Localization, ion channel regulation, and genetic interactions during abscisic acid signaling of the nuclear mRNA cap-binding protein, ABH1. Plant Physiol 130:1276–1287

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  98. Kong XX, Ma L, Yang LM, Chen Q, Xiang N, Yang YP, Hu XY (2014) Quantitative proteomics analysis reveals that the nuclear cap-binding complex proteins Arabidopsis CBP20 and CBP80 modulate the salt stress response. J Proteome Res 13:2495–2510

    Article  CAS  PubMed  Google Scholar 

  99. Jonas S, Izaurralde E (2013) The role of disordered protein regions in the assembly of decapping complexes and RNP granules. Genes Dev 27:2628–2641

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  100. Perea-Resa C, Carrasco-Lopez C, Catala R, Tureckova V, Novak O, Zhang WP, Sieburth L, Jimenez-Gomez JM, Salinas J (2016) The LSM1-7 complex differentially regulates Arabidopsis tolerance to abiotic stress conditions by promoting selective mRNA decapping. Plant Cell 28:505–520

    Google Scholar 

  101. Park SH, Chung PJ, Juntawong P, Bailey-Serres J, Kim YS, Jung H, Bang SW, Kim YK, Do Choi Y, Kim JK (2012) Posttranscriptional control of photosynthetic mRNA decay under stress conditions requires 3′ and 5′ untranslated regions and correlates with differential polysome association in rice. Plant Physiol 159:1111–1124

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  102. Xu J, Chua NH (2012) Dehydration stress activates Arabidopsis MPK6 to signal DCP1 phosphorylation. EMBO J 31:1975–1984

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  103. Stecker KE, Minkoff BB, Sussman MR (2014) Phosphoproteomic analyses reveal early signaling events in the osmotic stress response. Plant Physiol 165:1171–1187

    Article  CAS  Google Scholar 

  104. Brodersen P, Sakvarelidze-Achard L, Bruun-Rasmussen M, Dunoyer P, Yamamoto YY, Sieburth L, Voinnet O (2008) Widespread translational inhibition by plant miRNAs and siRNAs. Science 320:1185–1190

    Article  CAS  PubMed  Google Scholar 

  105. Skirycz A, Vandenbroucke K, Clauw P, Maleux K, De Meyer B, Dhondt S, Pucci A, Gonzalez N, Hoeberichts F, Tognetti VB et al (2011) Survival and growth of Arabidopsis plants given limited water are not equal. Nat Biotechnol 29:212–214

    Article  CAS  PubMed  Google Scholar 

  106. Verslues PE (2016) Time to grow: factors that control plant growth during mild to moderate drought stress. Plant Cell Environ 40(2):177–179. doi:10.1111/pce.12827

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

Research in the Verslues laboratory is supported by Academia Sinica and the Taiwan Ministry of Science and Technology. M.M.W. and G.L.C. have also received support from the Taiwan International Graduate Program.

Author information

Authors and Affiliations

  1. Institute of Plant and Microbial Biology, Academia Sinica, 128 Academia Road, Taipei, 11529, Taiwan

    Min May Wong, Geeng Loo Chong & Paul E. Verslues

  2. Molecular and Biological Agricultural Sciences Program, Taiwan International Graduate Program, Academia Sinica, Taipei, 11529, Taiwan

    Min May Wong & Geeng Loo Chong

  3. Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung, 40227, Taiwan

    Min May Wong & Geeng Loo Chong

  4. Biotechnology Center, National Chung-Hsing University, Taichung, 40227, Taiwan

    Paul E. Verslues

Authors
  1. Min May Wong
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Geeng Loo Chong
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Paul E. Verslues
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Paul E. Verslues .

Editor information

Editors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, Oklahoma, USA

    Ramanjulu Sunkar

Rights and permissions

Reprints and permissions

Copyright information

© 2017 Springer Science+Business Media LLC

About this protocol

Cite this protocol

Wong, M.M., Chong, G.L., Verslues, P.E. (2017). Epigenetics and RNA Processing: Connections to Drought, Salt, and ABA?. In: Sunkar, R. (eds) Plant Stress Tolerance. Methods in Molecular Biology, vol 1631. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7136-7_1

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-7136-7_1

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7134-3

  • Online ISBN: 978-1-4939-7136-7

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation