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Genome Editing of Rat

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Genome Editing in Animals

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1630))

Abstract

Many genetically engineered rat strains have been produced for biomedical research. The simple and quick production of knock-out and knock-in rats is currently possible using genome editing techniques incorporating zinc finger nuclease (ZFN), transcription activator-like effector nuclease (TALEN), or clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9. Genome-edited animals have been produced by the introduction of endonucleases into embryos using conventional microinjection and a new electroporation method named Technique for Animal Knockout system by Electroporation (TAKE). This chapter introduces the latest protocols for producing genetically engineered rats using genome editing.

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References

  1. Geurts AM, Cost GJ, Freyvert Y et al (2009) Knockout rats produced via embryo pronuclear microinjection of designed zinc finger nucleases. Science 325:433. doi:10.1126/science.1172447

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Tesson L, Usal C, Ménoret S et al (2011) Knockout rats generated by embryo microinjection of TALENs. Nat Biotechnol 29:695–696. doi:10.1038/nbt.1940

    Article  CAS  PubMed  Google Scholar 

  3. Li D, Qiu Z, Shao Y et al (2013) Heritable gene targeting in the mouse and rat using a CRISPR-Cas system. Nat Biotechnol 31:681–683. doi:10.1038/nbt.2661

    Article  CAS  PubMed  Google Scholar 

  4. Li W, Teng F, Li T et al (2013) Simultaneous generation and germline transmission of multiple gene mutations in rat using CRISPR-Cas systems. Nat Biotechnol 31:684–686. doi:10.1038/nbt.2652

    Article  CAS  PubMed  Google Scholar 

  5. Tong C, Li P, Wu NL et al (2010) Production of p53 gene knockout rats by homologous recombination in embryonic stem cells. Nature 467:211–213. doi:10.1038/nature09368

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Mashimo T, Takizawa A, Voigt B, Yoshimi K, Hiai H, Kuramoto T, Serikawa T (2010) Generation of knockout rats with X-linked severe combined immunodeficiency (X-SCID) using zinc-finger nucleases. PLoS One 5(1):e8870. doi:10.1371/journal.pone.0008870

    Article  PubMed  PubMed Central  Google Scholar 

  7. Sakuma T, Ochiai H, Kaneko T, Mashimo T, Tokumasu D, Sakane Y, Suzuki K, Miyamoto T, Sakamoto N, Matsuura S, Yamamoto T (2013) Repeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity. Sci Rep 3:3379. doi:10.1038/srep03379

    Article  PubMed  PubMed Central  Google Scholar 

  8. Yoshimi K, Kaneko T, Voigt B et al (2014) Allele-specific genome editing and correction of disease-associated phenotypes in rats using the CRISPR-Cas platform. Nat Commun 5:4240. doi:10.1038/ncomms5240

    CAS  PubMed  PubMed Central  Google Scholar 

  9. Yoshimi K, Kunihiro Y, Kaneko T et al (2016) ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes. Nat Commun 7:10431. doi:10.1038/ncomms10431

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Kaneko T, Sakuma T, Yamamoto T et al (2014) Simple knockout by electroporation of engineered endonucleases into intact rat embryos. Sci Rep 4:6382. doi:10.1038/srep06382

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Kaneko T, Mashimo T (2015) Simple genome editing of rodent intact embryos by electroporation. PLoS One 10:e0142755. doi:10.1371/journal.pone.0142755

    Article  PubMed  PubMed Central  Google Scholar 

  12. Taketsuru H, Kaneko T (2013) Efficient collection and cryopreservation of embryos in F344 strain inbred rats. Cryobiology 67:230–234. doi:10.1016/j.cryobiol.2013.07.004

    Article  CAS  PubMed  Google Scholar 

  13. Mashimo T, Kaneko T, Sakuma T et al (2013) Efficient gene targeting by TAL effector nucleases coinjected with exonucleases in zygotes. Sci Rep 3:1253. doi:10.1038/srep01253

    Article  PubMed  PubMed Central  Google Scholar 

  14. Sung YH, Kim JM, Kim HT et al (2014) Highly efficient gene knockout in mice and zebrafish with RNA-guided endonucleases. Genome Res 24:125–131. doi:10.1101/gr.163394.113

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Author information

Authors and Affiliations

  1. Division of Science and Engineering, Graduate School of Arts and Science, Iwate University, 4-3-5 Ueda, Morioka, Iwate, 020-8551, Japan

    Takehito Kaneko

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  1. Takehito Kaneko
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Correspondence to Takehito Kaneko .

Editor information

Editors and Affiliations

  1. Laboratory of Genome Science Biosignal Genome Resource Center Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Gunma, Japan

    Izuho Hatada

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Kaneko, T. (2017). Genome Editing of Rat. In: Hatada, I. (eds) Genome Editing in Animals. Methods in Molecular Biology, vol 1630. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7128-2_9

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  • DOI: https://doi.org/10.1007/978-1-4939-7128-2_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7127-5

  • Online ISBN: 978-1-4939-7128-2

  • eBook Packages: Springer Protocols

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