Abstract
CRISPR/Cas9 is a novel method that has become the most widely used genome editing technology around the world. Its widespread adoption is largely due to its simplicity and easy of use. Here, we introduce the construction of vectors and genome editing of the target gene in cells expressing the CRISPR/Cas9 system.
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Acknowledgments
This work was supported by grants from the Basic Science and Platform Technology Program for Innovative Biological Medicine from the Ministry of Education, Culture, Sports, Science, and Technology, Japan (MEXT), and The Japan Agency for Medical Research and Development (AMED) to I.H.
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Hatada, I., Horii, T. (2017). CRISPR/Cas9. In: Hatada, I. (eds) Genome Editing in Animals. Methods in Molecular Biology, vol 1630. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7128-2_3
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DOI: https://doi.org/10.1007/978-1-4939-7128-2_3
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7127-5
Online ISBN: 978-1-4939-7128-2
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