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Current Overview of TALEN Construction Systems

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Genome Editing in Animals

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1630))

Abstract

Transcription activator-like effector (TALE) nuclease (TALEN) is the second-generation genome editing tool consisting of TALE protein containing customizable DNA-binding repeats and nuclease domain of FokI enzyme. Each DNA-binding repeat recognizes one base of double-strand DNA, and functional TALEN can be created by a simple modular assembly of these repeats. To easily and efficiently assemble the highly repetitive DNA-binding repeat arrays, various construction systems such as Golden Gate assembly, serial ligation, and ligation-independent cloning have been reported. In this chapter, we summarize the current situation of these systems and publically available reagents and protocols, enabling optimal selection of best suited systems for every researcher who wants to utilize TALENs in various research fields.

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Acknowledgments

This work was partly supported by the Japan Society for the Promotion of Science (16 K18478 to T.S.).

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Authors and Affiliations

  1. Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima, Hiroshima, 739-8526, Japan

    Tetsushi Sakuma & Takashi Yamamoto

Authors
  1. Tetsushi Sakuma
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  2. Takashi Yamamoto
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Corresponding authors

Correspondence to Tetsushi Sakuma or Takashi Yamamoto .

Editor information

Editors and Affiliations

  1. Laboratory of Genome Science Biosignal Genome Resource Center Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Gunma, Japan

    Izuho Hatada

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Sakuma, T., Yamamoto, T. (2017). Current Overview of TALEN Construction Systems. In: Hatada, I. (eds) Genome Editing in Animals. Methods in Molecular Biology, vol 1630. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7128-2_2

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  • DOI: https://doi.org/10.1007/978-1-4939-7128-2_2

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7127-5

  • Online ISBN: 978-1-4939-7128-2

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