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Genome Editing of the Ascidian Ciona intestinalis with TALE Nuclease

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Genome Editing in Animals

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1630))

Abstract

The ascidian Ciona intestinalis is an important model animal for studying developmental mechanisms for constructing the chordate body. Although molecular and embryological techniques for manipulating Ciona genes were developed a long time ago, recent achievements of genome editing in this animal have innovated functional analyses of genes in Ciona. Particularly, knockout of genes in the G0 generation coupled with tissue-specific expression of TALENs enables us to rapidly address gene functions that were difficult using previous methods.

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Acknowledgments

We would like to thank Drs. Hiroshi Ochiai, Tetsushi Sakuma, and Takashi Yamamoto for their help to support our researches. This study was supported by Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (JSPS) and Ministry of Education, Culture, Sports, Science and Technology (MEXT) to Y.S. Y.S. was supported by the Toray Science and Technology Grant. Further support was provided by grants from the National Bioresource Project.

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Authors and Affiliations

  1. Shimoda Marine Research Center, University of Tsukuba, 5-10-1, Shimoda, Shizuoka, 415-0025, Japan

    Yasunori Sasakura, Keita Yoshida & Nicholas Treen

Authors
  1. Yasunori Sasakura
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  2. Keita Yoshida
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  3. Nicholas Treen
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Corresponding author

Correspondence to Yasunori Sasakura .

Editor information

Editors and Affiliations

  1. Laboratory of Genome Science Biosignal Genome Resource Center Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Gunma, Japan

    Izuho Hatada

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Sasakura, Y., Yoshida, K., Treen, N. (2017). Genome Editing of the Ascidian Ciona intestinalis with TALE Nuclease. In: Hatada, I. (eds) Genome Editing in Animals. Methods in Molecular Biology, vol 1630. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7128-2_19

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  • DOI: https://doi.org/10.1007/978-1-4939-7128-2_19

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7127-5

  • Online ISBN: 978-1-4939-7128-2

  • eBook Packages: Springer Protocols

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