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Applications of Amine-Reactive Tandem Mass Tags (TMT) in Human Neuroproteomics

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Current Proteomic Approaches Applied to Brain Function

Part of the book series: Neuromethods ((NM,volume 127))

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Abstract

Neuroproteomics is a complex field of life sciences due to the high complexity of the brain. This area comprises different pathophysiological conditions such as normal neurodevelopment, neurovascular disorders, and neurodegenerative disorders. A massive amount of studies have been performed using proteomics to increase the knowledge in this topic. However, there are still a lot more to explore. The most common proteomic techniques for investigating the different stages and conditions in neurodevelopment and diseases have mainly been based on two-dimensional gel electrophoresis (2-DE). More recently, the use of amine-reactive tandem mass tags (TMT) has also contributed to increase the understanding of the brain and associated disorders. The TMT can simultaneously compare up to ten samples and is compatible with a variety of biological samples. The proteins are labeled, pooled and co-eluted, and analyzed by LC-MS/MS. The multiplexing allows different designs and comparisons between the samples. Therefore the method is highly recommendable for, e.g., biomarker discovery in the neuroproteomic field. In this chapter the TMT 10-plex method will be detailed for use with three different brain proximal samples: cerebrospinal fluid (CSF), brain tissue, and neurons.

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Author notes

  1. Linnéa Lagerstedt and Leire Azurmendi contributed equally to this work.

Authors and Affiliations

  1. Translational Biomarker Group, Human Protein Sciences Department, Faculty of Medicine, Geneva University, 1 Michel Servet, 1211, Geneva 4, Switzerland

    Linnéa Lagerstedt, Leire Azurmendi & Jean-Charles Sanchez

Authors
  1. Linnéa Lagerstedt
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  2. Leire Azurmendi
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  3. Jean-Charles Sanchez
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Corresponding author

Correspondence to Jean-Charles Sanchez .

Editor information

Editors and Affiliations

  1. Clinical Neuroproteomics Unit, Navarrabiomed, Navarra Health Department, Public University of Navarra, Proteored-Institute of Health Carlos III (ISCIII), Navarra Institute for Health Research (IdiSNA), Pamplona, Spain

    Enrique SantamarĂ­a

  2. Clinical Neuroproteomics Unit, Navarrabiomed, Navarra Health Department, Public University of Navarra, Proteored-Institute of Health Carlos III (ISCIII), Navarra Institute for Health Research (IdiSNA), Pamplona, Spain

    JoaquĂ­n FernĂ¡ndez-Irigoyen

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Lagerstedt, L., Azurmendi, L., Sanchez, JC. (2017). Applications of Amine-Reactive Tandem Mass Tags (TMT) in Human Neuroproteomics. In: SantamarĂ­a, E., FernĂ¡ndez-Irigoyen, J. (eds) Current Proteomic Approaches Applied to Brain Function. Neuromethods, vol 127. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7119-0_2

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  • DOI: https://doi.org/10.1007/978-1-4939-7119-0_2

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7118-3

  • Online ISBN: 978-1-4939-7119-0

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