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Proteomic Analysis of Extracellular Vesicles in Neurological Diseases

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Current Proteomic Approaches Applied to Brain Function

Part of the book series: Neuromethods ((NM,volume 127))

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Abstract

Extracellular vesicles (EVs) play a key role in cell-to-cell communication carrying lipids, genetic material, and proteins, producing changes in their target cells. In the last years, EVs have been a hot topic owning to their potential source of biomarkers in many diseases. The search for new biomarkers of central nervous diseases is limited due to the difficulty in accessing to biopsies or complications after obtaining cerebrospinal fluid. In this sense, the study of EVs obtained from blood is proposed as a source of surrogate biomarkers of easy access. One of the most studied components of EVs as biomarkers is their protein cargo. In this chapter we present the main protocols for EV isolation from blood and urine samples, these being based on differential centrifugation and commercial kits for polymeric precipitation. Subsequently, the protocol for a proteomic characterization of plasma-derived EVs is also detailed.

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Author information

Authors and Affiliations

  1. Immunology Unit, Donostia University Hospital, San Sebastián, Spain

    Matías Sáenz-Cuesta

  2. Multiple Sclerosis Group, Biodonostia Health Research Institute, Neuroscience Area, Paseo Dr. Begiristain S/N, Donostia-San Sebastián, 20014, Guipúzcoa, Spain

    Matías Sáenz-Cuesta & David Otaegui

  3. Clinical Neuroproteomics Unit, Navarrabiomed, Navarra Health Department, Public University of Navarra, Proteored-Institute of Health Carlos III (ISCIII), Navarra Institute for Health Research (IdiSNA), Pamplona, Spain

    Enrique Santamaría & Joaquin Fernández-Irigoyen

Authors
  1. Matías Sáenz-Cuesta
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  2. Enrique Santamaría
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  3. Joaquin Fernández-Irigoyen
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  4. David Otaegui
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Corresponding author

Correspondence to David Otaegui .

Editor information

Editors and Affiliations

  1. Clinical Neuroproteomics Unit, Navarrabiomed, Navarra Health Department, Public University of Navarra, Proteored-Institute of Health Carlos III (ISCIII), Navarra Institute for Health Research (IdiSNA), Pamplona, Spain

    Enrique Santamaría

  2. Clinical Neuroproteomics Unit, Navarrabiomed, Navarra Health Department, Public University of Navarra, Proteored-Institute of Health Carlos III (ISCIII), Navarra Institute for Health Research (IdiSNA), Pamplona, Spain

    Joaquín Fernández-Irigoyen

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Sáenz-Cuesta, M., Santamaría, E., Fernández-Irigoyen, J., Otaegui, D. (2017). Proteomic Analysis of Extracellular Vesicles in Neurological Diseases. In: Santamaría, E., Fernández-Irigoyen, J. (eds) Current Proteomic Approaches Applied to Brain Function. Neuromethods, vol 127. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7119-0_15

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  • DOI: https://doi.org/10.1007/978-1-4939-7119-0_15

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7118-3

  • Online ISBN: 978-1-4939-7119-0

  • eBook Packages: Springer Protocols

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