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Modeling the Ebolavirus Life Cycle with Transcription and Replication-Competent Viruslike Particle Assays

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Book cover Ebolaviruses

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1628))

Abstract

Ebolaviruses are the causative agent of a severe hemorrhagic fever with high case fatality rates, for which no approved specific therapy is available. As biosafety level 4 (BSL4) agents, work with live ebolaviruses is restricted to maximum containment laboratories. Transcription and replication-competent viruslike particle (trVLP) systems are reverse genetics-based life cycle modeling systems that allow researchers to model virtually the entire ebolavirus life cycle outside of a maximum containment laboratory. These systems can be used to dissect the virus life cycle, and thus increase our understanding of virus biology, as well as for more applied uses such as the screening and development of novel antivirals, and thus represent powerful tools for work on ebolaviruses.

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Author information

Authors and Affiliations

  1. Institute for Virology, Philipps University Marburg, Marburg, Germany

    Nadine Biedenkopf

  2. Friedrich-Loeffler-Institut, Greifswald - Insel Riems, Germany

    Thomas Hoenen

Authors
  1. Nadine Biedenkopf
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  2. Thomas Hoenen
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Corresponding author

Correspondence to Thomas Hoenen .

Editor information

Editors and Affiliations

  1. Friedrich-Loeffler-Institut, Greifswald - Insel Riems, Germany

    Thomas Hoenen

  2. Friedrich-Loeffler-Institut, Greifswald - Insel Riems, Germany

    Allison Groseth

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Biedenkopf, N., Hoenen, T. (2017). Modeling the Ebolavirus Life Cycle with Transcription and Replication-Competent Viruslike Particle Assays. In: Hoenen, T., Groseth, A. (eds) Ebolaviruses. Methods in Molecular Biology, vol 1628. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7116-9_9

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  • DOI: https://doi.org/10.1007/978-1-4939-7116-9_9

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7115-2

  • Online ISBN: 978-1-4939-7116-9

  • eBook Packages: Springer Protocols

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