Abstract
Serological methods such as the enzyme-linked immunosorbent assay (ELISA) and virus neutralization test are fundamental tools used in diagnosis, seroepidemiological studies of filovirus transmission/prevalence, and the evaluation of vaccine immunogenicity and potential therapeutic antibodies. Filoviruses have a single transmembrane glycoprotein (GP), which is the only known target of neutralizing antibodies. Here we describe serological methods to quantify filovirus GP-specific antibodies.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsSpringer Nature is developing a new tool to find and evaluate Protocols. Learn more
References
Takada A, Robison C, Goto H, Sanchez A, Murti KG, Whitt MA, Kawaoka Y (1997) A system for functional analysis of Ebola virus glycoprotein. Proc Natl Acad Sci U S A 94:14764–14769
Wool-Lewis RJ, Bates P (1998) Characterization of Ebola virus entry by using pseudotyped viruses: identification of receptor-deficient cell lines. J Virol 72:3155–3160
Takada A, Feldmann H, Ksiazek TG, Kawaoka Y (2003) Antibody-dependent enhancement of Ebola virus infection. J Virol 77:7539–7544
Takada A, Kawaoka Y (2003) Antibody-dependent enhancement of viral infection: molecular mechanisms and in vivo implications. Rev Med Virol 13:387–398
Takada A, Ebihara H, Feldmann H, Geisbert TW, Kawaoka Y (2007) Epitopes required for antibody-dependent enhancement of Ebola virus infection. J Infect Dis 196(Suppl 2):S347–S356
Nakayama E, Tomabechi D, Matsuno K, Kishida N, Yoshida R, Feldmann H, Takada A (2011) Antibody-dependent enhancement of Marburg virus infection. J Infect Dis 204(Suppl 3):S978–S985
Nakayama E, Yokoyama A, Miyamoto H, Igarashi M, Kishida N, Matsuno K, Marzi A, Feldmann H, Ito K, Saijo M, Takada A (2010) Enzyme-linked immunosorbent assay for detection of filovirus species-specific antibodies. Clin Vaccine Immunol 17:1723–1728
Maruyama J, Miyamoto H, Kajihara M, Ogawa H, Maeda K, Sakoda Y, Yoshida R, Takada A (2014) Characterization of the envelope glycoprotein of a novel filovirus, Lloviu virus. J Virol 88:99–109
Takada A, Watanabe S, Okazaki K, Kida H, Kawaoka Y (2001) Infectivity-enhancing antibodies to Ebola virus glycoprotein. J Virol 75:2324–2330
Acknowledgments
This work was supported by the Agency for Medical Research and Development (AMED) and Japan International Cooperation Agency (JICA) within the framework of the Science and Technology Research Partnership for Sustainable Development (SATREPS), the Japan Initiative for Global Research Network on Infectious Diseases (J-GRID), and a Grant-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology (MEXT).
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer Science+Business Media LLC
About this protocol
Cite this protocol
Furuyama, W., Miyamoto, H., Yoshida, R., Takada, A. (2017). Quantification of Filovirus Glycoprotein-Specific Antibodies. In: Hoenen, T., Groseth, A. (eds) Ebolaviruses. Methods in Molecular Biology, vol 1628. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7116-9_25
Download citation
DOI: https://doi.org/10.1007/978-1-4939-7116-9_25
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7115-2
Online ISBN: 978-1-4939-7116-9
eBook Packages: Springer Protocols