Abstract
In this chapter, we describe a Western blot assay for the successful detection of apoptosis in Ebola virus (EBOV)-infected cells. The protocol includes all steps from cell culture, infection of cells, generation of lysates, and analysis using Western blot to detect caspase cleavage as marker of apoptosis.
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Acknowledgments
The authors are grateful to J.R. Pacheco (Boston University) for critical reading of the manuscript. The work was performed in the lab of Dr. Elke Mühlberger at the Boston University School of Medicine, Department of Microbiology and was funded by the National Institute of Allergy and Infectious Disease (NIAID) of the National Institutes of Health (NIH) under award numbers UC6 AI058618 and U01 AI082954.
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Olejnik, J., Nelson, E.V. (2017). Analyzing Apoptosis Induction and Evasion in Ebola Virus-Infected Cells. In: Hoenen, T., Groseth, A. (eds) Ebolaviruses. Methods in Molecular Biology, vol 1628. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7116-9_18
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DOI: https://doi.org/10.1007/978-1-4939-7116-9_18
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