Fibrosis pp 91-116 | Cite as

Mouse and Rat Models of Induction of Hepatic Fibrosis and Assessment of Portal Hypertension

  • Sabine Klein
  • Robert Schierwagen
  • Frank Erhard Uschner
  • Jonel TrebickaEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1627)


Portal hypertension either develops due to progressive liver fibrosis or is the consequence of vascular liver diseases such as portal vein thrombosis or non-cirrhotic portal hypertension. This chapter focuses on different rodent models of liver fibrosis with portal hypertension and also in few non-cirrhotic portal hypertension models. Importantly, after the development of portal hypertension, the proper assessment of drug effects in the portal and systemic circulation should be discussed. The last part of the chapter is dedicated in these techniques to assess the in vivo hemodynamics and the ex vivo techniques of the isolated liver perfusion and vascular contractility.

Key words

Portal hypertension Bile duct ligation Carbon tetrachloride Methionine-choline-deficient diet High-fat diet Partial portal vein ligation Colored microsphere technique Isolated in situ liver perfusion Aortic ring contraction 



The Laboratory is supported by grants from the Deutsche Forschungsgemeinschaft (SFB TRR57 to P18), the H. J. & W. Hector Stiftung, Ernst-Bertha-Grimmke-Stiftung (6/15) and European Union’s Horizon 2020 research and innovation programme (No 668031).


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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Sabine Klein
    • 1
  • Robert Schierwagen
    • 2
  • Frank Erhard Uschner
    • 3
  • Jonel Trebicka
    • 4
    Email author
  1. 1.Laboratory for Liver Fibrosis and Portal Hypertension, Department of Internal Medicine IUniversity of Clinic BonnBonnGermany
  2. 2.European Foundation for the Study of Chronic Liver FailureBarcelonaSpain
  3. 3.Faculty of Health SciencesUniversity of Southern DenmarkOdenseDenmark
  4. 4.Institute for Bioengineering of CataloniaBarcelonaSpain

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