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Transplanting Human Skin Grafts onto Nude Mice to Model Skin Scars

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Fibrosis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1627))

Abstract

Hypertrophic scar (HTS) is a common outcome of deep dermal wound healing mainly followed mechanical, chemical, and thermal injuries in the skin. Because of the lack of the most effective prevention and treatment, it is particularly important to establish an ideal dermal animal model for improving the understanding of the pathogenesis and exploring therapeutic approaches of HTS. Compared to other dermal fibrotic animal models in rabbits, red Duroc pigs, guinea pigs, rats, and mice, the approach that uses normal human split-thickness skin grafted onto nude or other immunodeficient mice which develop scars that resemble human HTS offers the advantages of lower cost, easier manipulation, and shorter research period. In this chapter, we will introduce the detailed procedures to create the ideal dermal fibrotic mouse model.

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Acknowledgment

This work was supported by Firefighter’s Burn Trust Fund, Edmonton Civic Employees Research Awards, and University of Alberta Hospital Foundation.

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Authors and Affiliations

  1. Wound Healing Research Group, Division of Plastic and Reconstructive Surgery, Department of Surgery, University of Alberta, Edmonton, AB, Canada

    Jie Ding M.D., PhD. & Edward E. Tredget

  2. Division of Critical Care Medicine, Department of Surgery, University of Alberta, Edmonton, AB, Canada

    Edward E. Tredget

Authors
  1. Jie Ding M.D., PhD.
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  2. Edward E. Tredget
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Corresponding author

Correspondence to Edward E. Tredget .

Editor information

Editors and Affiliations

  1. Department of Dermatology, University of Michigan Medical School, Ann Arbor, Michigan, USA

    Laure Rittié

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Ding, J., Tredget, E.E. (2017). Transplanting Human Skin Grafts onto Nude Mice to Model Skin Scars. In: Rittié, L. (eds) Fibrosis. Methods in Molecular Biology, vol 1627. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7113-8_5

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  • DOI: https://doi.org/10.1007/978-1-4939-7113-8_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7112-1

  • Online ISBN: 978-1-4939-7113-8

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