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Histological and Electron Microscope Staining for the Identification of Elastic Fiber Networks

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Book cover Fibrosis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1627))

Abstract

Elastic fibers are a major component of the extracellular matrix and are present in many tissues. Routine histology and standard electron microscopy procedures often do not allow for clear identification of elastic fibers making their organization and ultrastructure difficult to study. In this paper, we describe staining methods and procedures to enhance the contrast of elastin at both the light and electron microscope levels.

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References

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Acknowledgments

This work was supported by NSERC grant 355710 and funding from the Faculty of Medicine, McGill University to E.C.D.

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Correspondence to Elaine C. Davis .

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Davis, E.C., Li, L. (2017). Histological and Electron Microscope Staining for the Identification of Elastic Fiber Networks. In: Rittié, L. (eds) Fibrosis. Methods in Molecular Biology, vol 1627. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7113-8_25

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  • DOI: https://doi.org/10.1007/978-1-4939-7113-8_25

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7112-1

  • Online ISBN: 978-1-4939-7113-8

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