Isolation of Live Fibroblasts by Fluorescence-Activated Cell Sorting
Flow cytometry is a powerful tool in cell biology in that it allows real-time characterization of cellular phenotypes. Additionally, through the process of fluorescence-activated cell sorting (FACS), living cells can be isolated for future in vitro experiments, including single cell analysis. Here we describe the isolation of live fibroblasts from the dermis of the skin in mice using FACS. This method circumvents the need for ex vivo expansion in cell culture, which can alter phenotypic and functional characteristics of cells.
Key wordsFibroblasts Skin Flow cytometry Fluorescence-activated cell sorting Dermis Dermal tissue harvest Live cell isolation
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