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Vaccines for Invasive Fungal Infections pp 225–235Cite as

Vaccination with Phage-Displayed Antigenic Epitope

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1625))

Abstract

Phage-displayed antigenic epitope is able to induce both humoral and cell-mediated immune responses, which could be used as an delivery system to design vaccines without compromising safety and tolerability. Here, we describe the vaccination with phage-displayed antigenic epitope (M13 phage-displayed SLAQVKYTSASSI) against Candida albicans infection in BALB/c mice by this method.

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References

  1. Smith GP (1985) Filamentous fusion phage: novel expression vectors that display cloned antigens on the virion surface. Science 228(4705):1315–1317

    Article  CAS  PubMed  Google Scholar 

  2. Marvin D, Hale R, Nave C, Citterich MH (1994) Molecular models and structural comparisons of native and mutant class I filamentous bacteriophages: Ff (fd, f1, M13), If1 and IKe. J Mol Biol 235(1):260–286

    Article  CAS  PubMed  Google Scholar 

  3. Scott JK, Smith GP (1990) Searching for peptide ligands with an epitope library. Science 249(4967):386–390

    Article  CAS  PubMed  Google Scholar 

  4. Fuh G, Sidhu SS (2000) Efficient phage display of polypeptides fused to the carboxy-terminus of the M13 gene-3 minor coat protein. FEBS Lett 480(2–3):231–234

    Article  CAS  PubMed  Google Scholar 

  5. Weiss GA, Roth TA, Baldi PF, Sidhu SS (2003) Comprehensive mutagenesis of the C-terminal domain of the M13 gene-3 minor coat protein: the requirements for assembly into the bacteriophage particle. J Mol Biol 332(4):777–782

    Article  CAS  PubMed  Google Scholar 

  6. Koff WC, Johnson PR, Watkins DI, Burton DR, Lifson JD, Hasenkrug KJ, McDermott AB, Schultz A, Zamb TJ, Boyle R (2006) HIV vaccine design: insights from live attenuated SIV vaccines. Nat Immunol 7(1):19–24

    Article  CAS  PubMed  Google Scholar 

  7. Bachmann MF, Jennings GT (2010) Vaccine delivery: a matter of size, geometry, kinetics and molecular patterns. Nat Rev Immunol 10(11):787–796

    Article  CAS  PubMed  Google Scholar 

  8. Wang Y, Su Q, Dong S, Shi H, Gao X, Wang L (2014) Hybrid phage displaying SLAQVKYTSASSI induces protection against Candida albicans challenge in BALB/c mice. Hum Vaccin Immunother 10(4):1057–1063

    Article  PubMed  PubMed Central  Google Scholar 

  9. Del Pozzo G, Mascolo D, Sartorius R, Citro A, Barba P, D'Apice L, De Berardinis P (2010) Triggering DTH and CTL activity by fd filamentous bacteriophages: role of CD4+ T cells in memory responses. Biomed Res Int 2010:894971

    Google Scholar 

  10. Prisco A, De Berardinis P (2012) Filamentous bacteriophage fd as an antigen delivery system in vaccination. Int J Mol Sci 13(4):5179–5194

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Jepson CD, March JB (2004) Bacteriophage lambda is a highly stable DNA vaccine delivery vehicle. Vaccine 22(19):2413–2419

    Article  CAS  PubMed  Google Scholar 

  12. Fehr T, Skrastina D, Pumpens P, Zinkernagel RM (1998) T cell-independent type I antibody response against B cell epitopes expressed repetitively on recombinant virus particles. Proc Natl Acad Sci 95(16):9477–9481

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. di Marzo VF, Willis AE, Boyer-Thompson C, Appella E, Perham RN (1994) Structural mimicry and enhanced immunogenicity of peptide epitopes displayed on filamentous bacteriophage. The V3 loop of HIV-1 gp120. J Mol Biol 243(2):167–172

    Article  Google Scholar 

  14. Vonk AG, Netea MG, van der Meer JW, Kullberg BJ (2006) Host defence against disseminated Candida albicans infection and implications for antifungal immunotherapy. Expert Opin Biol Ther 6(9):891–903

    Article  CAS  PubMed  Google Scholar 

  15. Cassone A, Casadevall A (2012) Recent progress in vaccines against fungal diseases. Curr Opin Microbiol 15(4):427–433

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Malik P, Terry TD, Gowda LR, Langara A, Petukhov SA, Symmons MF, Welsh LC, Marvin DA, Perham RN (1996) Role of capsid structure and membrane protein processing in determining the size and copy number of peptides displayed on the major coat protein of filamentous bacteriophage. J Mol Biol 260(1):9–21

    Article  CAS  PubMed  Google Scholar 

  17. Schägger H, Von Jagow G (1987) Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem 166(2):368–379

    Article  PubMed  Google Scholar 

  18. Matthews RC, Burnie JP, Tabaqchali S (1987) Isolation of immunodominant antigens from sera of patients with systemic candidiasis and characterization of serological response to Candida albicans. J Clin Microbiol 25(2):230–237

    CAS  PubMed  PubMed Central  Google Scholar 

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Author information

Authors and Affiliations

  1. Jilin Provincial Key Laboratory on Molecular and Chemical Genetic, The Second Hospital of Jilin University, 218 Ziqiang Street, Changchun, 130041, China

    Yicun Wang

  2. Institute of Genetics and Cytology, School of Life Sciences, Northeast Normal University, 5268 Renmin Street, Changchun, 130042, China

    Li Wang

Authors
  1. Yicun Wang
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  2. Li Wang
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Corresponding author

Correspondence to Li Wang .

Editor information

Editors and Affiliations

  1. Department of Molecular Immunology, Beckman Research Institute of City of Hope, Duarte, California, USA

    Markus Kalkum

  2. Department of Molecular Immunology, Beckman Research Institute of City of Hope, Duarte, California, USA

    Margarita Semis

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Wang, Y., Wang, L. (2017). Vaccination with Phage-Displayed Antigenic Epitope. In: Kalkum, M., Semis, M. (eds) Vaccines for Invasive Fungal Infections. Methods in Molecular Biology, vol 1625. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7104-6_15

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  • DOI: https://doi.org/10.1007/978-1-4939-7104-6_15

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7103-9

  • Online ISBN: 978-1-4939-7104-6

  • eBook Packages: Springer Protocols

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