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The Bacterial Nucleoid pp 75–84Cite as

Generation and Analysis of Chromosomal Contact Maps of Bacteria

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1624))

Abstract

This methods article described a protocol aiming at generating chromosome contact maps of bacterial species using a genome-wide derivative of the chromosome conformation capture (3C) technique. The approach is readily applicable on a broad variety of gram + and gram-bacterial species. It describes and addresses known caveats and technicalities associated with the technique, and should be of interest to any laboratory interested to perform a multiscale analysis of the genome structure of its species of interest.

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Acknowledgments

This research was supported by funding to R.K. from the European Research Council under the seventh Framework Program (FP7/2007-2013, ERC grant agreement 260822) and from Agence Nationale pour la Recherche (HiResBaCS ANR-15-CE11-0023).

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Authors and Affiliations

  1. Groupe Régulation Spatiale des Génomes, Département Génomes et Génétique, Institut Pasteur, 28 rue du Docteur Roux, 75724, Paris Cedex 15, France

    Martial Marbouty & Romain Koszul

  2. CNRS, UMR 3525, 75724, Paris Cedex 15, France

    Martial Marbouty & Romain Koszul

  3. Centre de Bioinformatique, Biostatistique et Biologie Intégrative, USR 3756, Institut Pasteur, Paris Cedex 15, 75724, Paris Cedex 15, France

    Martial Marbouty & Romain Koszul

Authors
  1. Martial Marbouty
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  2. Romain Koszul
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Corresponding authors

Correspondence to Martial Marbouty or Romain Koszul .

Editor information

Editors and Affiliations

  1. UMR CNRS 7241, INSERM U1050, CIRB, Collège de France, Paris, France

    Olivier Espéli

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Marbouty, M., Koszul, R. (2017). Generation and Analysis of Chromosomal Contact Maps of Bacteria. In: Espéli, O. (eds) The Bacterial Nucleoid. Methods in Molecular Biology, vol 1624. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7098-8_7

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  • DOI: https://doi.org/10.1007/978-1-4939-7098-8_7

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7097-1

  • Online ISBN: 978-1-4939-7098-8

  • eBook Packages: Springer Protocols

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