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Imaging the Cell Cycle of Pathogen E. coli During Growth in Macrophage

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The Bacterial Nucleoid

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1624))

Abstract

The study of the bacterial cell cycle at the single cell level can not only give insights on the fitness of the bacterial population but also reveal heterogeneous behavior. Typically, the DNA replication, the cell division, and the nucleoid conformation are appropriate representatives of the bacterial cell cycle. Because bacteria rapidly adapt their growth rate to environmental changes, the measure of cell cycle parameters gives valuable insights for the study of bacterial stress response or host–pathogen interactions. Here we describe methods to first introduce fluorescent fusion proteins and fluorescent tag within the chromosome of pathogenic bacteria to study these cell cycle steps; then to follow them within macrophages using a confocal spinning disk microscope.

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Authors and Affiliations

  1. CIRB, Collège de France, UMR CNRS 7241 INSERM U1050, 11 Place Marcelin Berthelot, 75005, Paris, France

    Gaëlle Demarre, Victoria Prudent & Olivier Espéli

Authors
  1. Gaëlle Demarre
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  2. Victoria Prudent
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  3. Olivier Espéli
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Corresponding authors

Correspondence to Gaëlle Demarre or Olivier Espéli .

Editor information

Editors and Affiliations

  1. UMR CNRS 7241, INSERM U1050, CIRB, Collège de France, Paris, France

    Olivier Espéli

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Demarre, G., Prudent, V., Espéli, O. (2017). Imaging the Cell Cycle of Pathogen E. coli During Growth in Macrophage. In: Espéli, O. (eds) The Bacterial Nucleoid. Methods in Molecular Biology, vol 1624. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7098-8_17

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  • DOI: https://doi.org/10.1007/978-1-4939-7098-8_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7097-1

  • Online ISBN: 978-1-4939-7098-8

  • eBook Packages: Springer Protocols

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