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Cytokine Expression by T Follicular Helper Cells

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Germinal Centers

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1623))

Abstract

T follicular helper (Tfh) cells are a specialized subset of CD4+ T cells located within temporary structures known as germinal centers (GC) formed within B cell follicles of secondary lymphoid organs. In the GC, Tfh cells facilitate the production of high-affinity antibodies through secretion of effector cytokines, such as IL-21 and IL-4, and through cell-to-cell interactions. The flow cytometric-based method described here allows the detection of intracellular cytokines within the Tfh population of secondary lymphoid organs (e.g., spleen, lymph nodes, and lymphoid nodules such as Peyer’s patches), enabling the study of Tfh responses to different stimuli in the context of immunity and autoimmunity.

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Author notes

  1. Christoph Jandl and Claudia Loetsch have equally contributed.

Authors and Affiliations

  1. Department of Immunology, Garvan Institute of Medical Research, 384 Victoria St., Darlinghurst, NSW, 2010, Australia

    Christoph Jandl, Claudia Loetsch & Cecile King Ph.D.

  2. St Vincent’s Clinical School, Department of Medicine, University of NSW, Sydney, NSW, 2010, Australia

    Christoph Jandl, Claudia Loetsch & Cecile King Ph.D.

Authors
  1. Christoph Jandl
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  2. Claudia Loetsch
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  3. Cecile King Ph.D.
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Corresponding author

Correspondence to Cecile King Ph.D. .

Editor information

Editors and Affiliations

  1. Immunity and Cancer Laboratory, The Francis Crick Institute, and King’s College London, London, United Kingdom

    Dinis Pedro Calado

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Jandl, C., Loetsch, C., King, C. (2017). Cytokine Expression by T Follicular Helper Cells. In: Calado, D. (eds) Germinal Centers. Methods in Molecular Biology, vol 1623. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7095-7_8

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  • DOI: https://doi.org/10.1007/978-1-4939-7095-7_8

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7094-0

  • Online ISBN: 978-1-4939-7095-7

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