Abstract
Dynamic imaging analyses of antigen-specific T–B interactions in germinal centers have advanced our understanding of the molecular mechanisms underlying affinity maturation and provided a wealth of information about how follicular helper T cells function in vivo. Here we describe a routine method to visualize fluorescence protein-expressing, antigen-specific T and B cells in germinal centers. The protocol for incorporating functional reporters or genetic perturbation of the T cells by retroviral transduction is also briefly described, using the FRET-based calcium reporter as an example.
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Acknowledgments
This work is supported in part by National Natural Science Foundation of China (grant No. 81330070 and 81425011). Photos of surgical procedures are courtesy of Jiacong Yan.
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Shih, C., Qi, H. (2017). Intravital Microscopy of T–B Cell Interactions in Germinal Centers. In: Calado, D. (eds) Germinal Centers. Methods in Molecular Biology, vol 1623. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7095-7_6
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DOI: https://doi.org/10.1007/978-1-4939-7095-7_6
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