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Tracking Plasma Cell Differentiation in Living Mice with Two-Photon Microscopy

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Germinal Centers

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1623))

Abstract

Due to the multitude of cell types involved in the differentiation of plasma cells during the germinal center reaction, and due to a lack of in vitro systems, which recapitulate germinal centers, the most suitable way to study plasma cell generation in germinal centers is in vivo. In this chapter we describe how to induce humoral immune responses to defined model antigens and how to visualize and track plasma cells and their interactions with other cells in the lymph nodes of living mice.

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Acknowledgement

We would like to thank Raluca Niesner for support with intravital microscopy and Robert Günther and Ralf Uecker for excellent technical assistance. A.E.H is supported by grants from the DFG: TRR130 TP17, HA5354/6-1 (FOR2165) HA5354/8-1 and EXC 257 NeuroCure.

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Author notes

  1. Authors Carolin Ulbricht and Randall L. Lindquist contributed equally to this work.

Authors and Affiliations

  1. Immune Dynamics and Intravital Microscopy, Charité Universitätsmedizin, Charitéplatz 1, 10117, Berlin, Germany

    Carolin Ulbricht & Anja E. Hauser

  2. Deutsches Rheumaforschungszentrum, Charitéplatz 1, 10117, Berlin, Germany

    Randall L. Lindquist, Laura Tech & Anja E. Hauser

Authors
  1. Carolin Ulbricht
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  2. Randall L. Lindquist
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  3. Laura Tech
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  4. Anja E. Hauser
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Corresponding author

Correspondence to Anja E. Hauser .

Editor information

Editors and Affiliations

  1. Immunity and Cancer Laboratory, The Francis Crick Institute, and King’s College London, London, United Kingdom

    Dinis Pedro Calado

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Ulbricht, C., Lindquist, R.L., Tech, L., Hauser, A.E. (2017). Tracking Plasma Cell Differentiation in Living Mice with Two-Photon Microscopy. In: Calado, D. (eds) Germinal Centers. Methods in Molecular Biology, vol 1623. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7095-7_3

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  • DOI: https://doi.org/10.1007/978-1-4939-7095-7_3

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7094-0

  • Online ISBN: 978-1-4939-7095-7

  • eBook Packages: Springer Protocols

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