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Analysis of the Germinal Center Reaction in Tissue Sections

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Germinal Centers

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1623))

Abstract

Germinal centers are short-lived microanatomical compartments with essential roles in adaptive immunity. These lymphoid structures can be identified in secondary lymphoid organs using both flow cytometry and immunohistological analyses, but only the latter provides useful architectural and spatial information. Here we describe how to use immunofluorescence and immunohistochemistry with specific antibodies to precisely highlight the cellular and architectural features of germinal centers, both in human and mouse secondary lymphoid organs, and to study their normal development and disturbance in disease.

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Acknowledgment

We wish to thank Carla Rossana Scalia, Rossella Gendusa for excellent technical assistance, Maddalena Maria Bolognesi for digital editing of multiplex images, and Franco Ferrario, Hong Yan and Qiong Shen for support and helpful insights.

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Authors and Affiliations

  1. Department of Oncological Sciences, Icahn School of Medicine at Mount Sinai, One Gustave L Levy Place, Box 1044A, New York, NY, 10029, USA

    David Dominguez-Sola

  2. The Tisch Cancer Institute & Precision Immunology Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA

    David Dominguez-Sola

  3. Dipartimento di Medicina e Chirurgia, Universitá degli Studi di Milano-Bicocca (UNIMIB), Via Cadore 48, 20900, Monza, Italy

    Giorgio Cattoretti

  4. Anatomia Patologica, Azienda Ospedaliera San Gerardo, Via Pergolesi 33, 20900, Monza, Italy

    Giorgio Cattoretti

Authors
  1. David Dominguez-Sola
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  2. Giorgio Cattoretti
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Corresponding authors

Correspondence to David Dominguez-Sola or Giorgio Cattoretti .

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Editors and Affiliations

  1. Immunity and Cancer Laboratory, The Francis Crick Institute, and King’s College London, London, United Kingdom

    Dinis Pedro Calado

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Dominguez-Sola, D., Cattoretti, G. (2017). Analysis of the Germinal Center Reaction in Tissue Sections. In: Calado, D. (eds) Germinal Centers. Methods in Molecular Biology, vol 1623. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7095-7_1

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  • DOI: https://doi.org/10.1007/978-1-4939-7095-7_1

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