Abstract
The oxidative burst or the production of reactive oxygen species (ROS) is a typical cellular response of both plants and animals to diverse abiotic and biotic stresses. Mainly, the (re-)active oxygen species include the superoxide anion (O2 −), hydrogen peroxide (H2O2), and the hydroxyl radical (OH•). Here, we outline the detection of extracellularly produced ROS in plant leaf pieces using a chemiluminescence-based bioassay with the luminol L-012 as a substrate being oxidized in the presence of ROS. Since this type of assay is in use in many laboratories, e.g., as a readout for activation of plant receptor kinases, we include a discussion on the interpretation of results and points addressing problems with the buffers at suboptimal pH values that negatively influence the chemiluminescence production.
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Acknowledgments
M.A. was supported by the “Deutsche Forschungsgemeinschaft” (AL1426/1-2), and U.F. was also supported by the German Federal Ministry of Education (BMBF–KBBE project 031A328 36). We thank Georg Felix for critical reading of the manuscript.
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Albert, M., Fürst, U. (2017). Quantitative Detection of Oxidative Burst upon Activation of Plant Receptor Kinases. In: Aalen, R. (eds) Plant Receptor Kinases. Methods in Molecular Biology, vol 1621. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7063-6_7
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DOI: https://doi.org/10.1007/978-1-4939-7063-6_7
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