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Plant Receptor Kinases pp 21–27Cite as

Expression of Plant Receptor Kinases in Yeast

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1621))

Abstract

The budding yeast Saccharomyces cerevisiae is a useful system to express recombinant proteins and analyze protein–protein interaction. Membrane-spanning proteins like plant receptor kinases find their way to the plasma membrane when expressed in yeast and seem to retain their structure and function. Here, we describe a general yeast DNA transformation procedure based on lithium acetate, salmon sperm DNA, and polyethylene glycol used to express recombinant proteins. Yeast cells expressing plant receptor kinases can be used for in vivo and in vitro studies of receptor function.

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Acknowledgments

This work was supported by grants to J.M. (UBACyT, PICT2012, PICT2014, and PICT2015).

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Authors and Affiliations

  1. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, “Dr. Héctor Torres” (INGEBI-CONICET), Vueltade Obligado 2490, C1428ADN, Buenos Aires, Argentina

    María Laura Barberini & Jorge P. Muschietti

Authors
  1. María Laura Barberini
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  2. Jorge P. Muschietti
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Corresponding author

Correspondence to Jorge P. Muschietti .

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Editors and Affiliations

  1. Department of Biosciences, University of Oslo, Oslo, Norway

    Reidunn Birgitta Aalen

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Barberini, M.L., Muschietti, J.P. (2017). Expression of Plant Receptor Kinases in Yeast. In: Aalen, R. (eds) Plant Receptor Kinases. Methods in Molecular Biology, vol 1621. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7063-6_2

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  • DOI: https://doi.org/10.1007/978-1-4939-7063-6_2

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7062-9

  • Online ISBN: 978-1-4939-7063-6

  • eBook Packages: Springer Protocols

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