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Plant Receptor Kinases pp 3–20Cite as

Expression of Plant Receptor Kinases in E. coli

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1621))

Abstract

Plant receptor kinases play diverse signaling roles in disease resistance and plant development. They represent a large plant gene family with over 600 members in Arabidopsis thaliana. While the functions of several members of the receptor kinase family have now been elucidated, a great proportion still remains uncharacterized. The structural and functional characterization of such plant receptor kinases may entail biochemical approaches that require access to purified protein, which can be made possible through heterologous protein expression. This chapter describes a strategy for expressing plant receptor kinases in E. coli, a bacterial host that has successfully been used to express and purify certain plant receptor kinase domains, some of which were subsequently used for biochemical assays. As full-length receptor-like kinases may be difficult to express, it is suggested to clone and express domains separately, after having identified domain borders using bioinformatics tools. A detailed cloning protocol is provided, as well as advice for testing expression efficiency and handling of expressed protein ending up in inclusion bodies.

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Author information

Authors and Affiliations

  1. Department of Biology, SBA School of Science and Engineering, Lahore University of Management Sciences, Lahore, 54792, Pakistan

    Moneeza Akbar Agha & Ahmed Jawaad Afzal

  2. Department of Plant, Soil and Agricultural Systems, Southern Illinois University, Carbondale, IL, 62901, USA

    David Lightfoot & Ahmed Jawaad Afzal

Authors
  1. Moneeza Akbar Agha
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  2. David Lightfoot
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  3. Ahmed Jawaad Afzal
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Corresponding author

Correspondence to Ahmed Jawaad Afzal .

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Editors and Affiliations

  1. Department of Biosciences, University of Oslo, Oslo, Norway

    Reidunn Birgitta Aalen

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Agha, M.A., Lightfoot, D., Afzal, A.J. (2017). Expression of Plant Receptor Kinases in E. coli . In: Aalen, R. (eds) Plant Receptor Kinases. Methods in Molecular Biology, vol 1621. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7063-6_1

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  • DOI: https://doi.org/10.1007/978-1-4939-7063-6_1

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7062-9

  • Online ISBN: 978-1-4939-7063-6

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