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Rapid Antibiotic Susceptibility Testing for Urinary Tract Infections

  • Anja Mezger
  • Mats Nilsson
  • Dan I. Andersson
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1616)

Abstract

Antibiotic susceptibility testing is important to guide clinicians in their choice of antibiotic used for treatment of bacterial infections. Current methods are time-consuming and more rapid alternatives are needed. Here, we describe a novel rapid method for antibiotic susceptibility testing which combines phenotypic and genotypic measurements. The use of padlock probes and rolling circle amplification allows for fast and precise determination of antibiotic susceptibilities as well as species identification.

Key words

Antibiotic susceptibility testing Urinary tract infections Padlock probes Rolling circle amplification 

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Broad Institute of MIT and HarvardCambridgeUSA
  2. 2.Department of Biological EngineeringMassachusetts Institute of TechnologyCambridgeUSA
  3. 3.Science for Life Laboratory, Department of Biochemistry and BiophysicsStockholm UniversitySolnaSweden
  4. 4.Department of Medical Biochemistry and MicrobiologyUppsala UniversityUppsalaSweden

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