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Multilocus Sequence Typing (MLST) for Cronobacter spp.

  • Susan Joseph
  • Stephen Forsythe
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1616)

Abstract

MLST is a molecular typing technique that involves the identification and clustering of bacterial isolates based on the partial sequence analysis of multiple housekeeping genes (generally seven) which are distributed across the length of the genome of the organism. The Cronobacter whole genus MLST scheme can be successfully used for an accurate species level identification and classification of this complex genus.

Key words

Cronobacter MLST Sequence type Clonal complex PubMLST Neonatal meningitis ST4 

References

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Institute of Dentistry, Barts and The London School of Medicine & DentistryQueen Mary University of LondonLondonUK
  2. 2.Pathogen Research Group, School of Science & TechnologyNottingham Trent UniversityNottinghamUK

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