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Identification of Lipoproteins Using Globomycin and Radioactive Palmitate

  • Nienke Buddelmeijer
Part of the Methods in Molecular Biology book series (MIMB, volume 1615)

Abstract

Bacterial lipoproteins are characterized by fatty acids that are covalently attached to their amino terminus via posttranslational modification in the cytoplasmic membrane. Three enzymatic steps are involved in the synthesis of mature triacylated lipoprotein: prolipoprotein converts into diacylglyceryl-prolipoprotein that in turn converts into apolipoprotein, which is finally converted into mature triacylated lipoprotein. Here we describe the detection of one of these intermediate forms of lipoprotein, diacylglyceryl-prolipoprotein, using 3H-palmitate labeling and inhibition by globomycin and detection by fluorography.

Key words

Tris-tricine gel electrophoresis Globomycin 3H-palmitate labeling Fluorography 

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Institut Pasteur, Biology and Genetics of the Bacterial Cell Wall UnitInserm Group AvenirParis cedex 15France

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