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Identification of Lipoproteins Using Globomycin and Radioactive Palmitate

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Bacterial Protein Secretion Systems

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1615))

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Abstract

Bacterial lipoproteins are characterized by fatty acids that are covalently attached to their amino terminus via posttranslational modification in the cytoplasmic membrane. Three enzymatic steps are involved in the synthesis of mature triacylated lipoprotein: prolipoprotein converts into diacylglyceryl-prolipoprotein that in turn converts into apolipoprotein, which is finally converted into mature triacylated lipoprotein. Here we describe the detection of one of these intermediate forms of lipoprotein, diacylglyceryl-prolipoprotein, using 3H-palmitate labeling and inhibition by globomycin and detection by fluorography.

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Authors and Affiliations

  1. Institut Pasteur, Biology and Genetics of the Bacterial Cell Wall Unit, Inserm Group Avenir, F-75724, Paris cedex 15, France

    Nienke Buddelmeijer

Authors
  1. Nienke Buddelmeijer
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Correspondence to Nienke Buddelmeijer .

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Editors and Affiliations

  1. Institut de Microbiologie de la Méditerranée, Aix-Marseille Univ - CNRS, Marseille, France

    Laure Journet

  2. Institut de Microbiologie de la Méditerranée, Aix-Marseille Univ - CNRS, Marseille, France

    Eric Cascales

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Buddelmeijer, N. (2017). Identification of Lipoproteins Using Globomycin and Radioactive Palmitate. In: Journet, L., Cascales, E. (eds) Bacterial Protein Secretion Systems. Methods in Molecular Biology, vol 1615. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7033-9_5

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  • DOI: https://doi.org/10.1007/978-1-4939-7033-9_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7031-5

  • Online ISBN: 978-1-4939-7033-9

  • eBook Packages: Springer Protocols

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