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Methods for Studying the Role of RAAS in the Modulation of Vascular Repair-Relevant Functions of Stem/Progenitor Cells

  • Yagna P. R. JarajapuEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1614)

Abstract

In recent years, previously unknown functions have been conferred to the RAAS and have been explored in mechanistic studies and disease models. Implication of bone marrow stem/progenitor cells in the cardiovascular protective or detrimental effects of RAAS is a prominent advancement because of the translational significance. Selected members of RAAS are now known to modulate migration, proliferation, and mobilization of bone marrow cells in response to ischemic insult, which are sensitive indicators of vascular repair-relevant functions. In this Chapter, protocols for most frequently used, in vitro, ex vivo, and in vivo assays to explore the potential of RAAS members to stimulate vascular repair-relevant functions of bone marrow stem/progenitor cells of human and murine origin.

Key words

CD34+ cells LSK cells Bone marrow Mobilization Flow cytometry Migration Proliferation 

Notes

Acknowledgments

The author wishes to acknowledge Mr. Shrinidh Joshi, and Mr. Goutham Vasam, for their contributions in refining some of the protocols described in this chapter.

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Department of Pharmaceutical SciencesCollege of Health Professions, North Dakota State UniversityFargoUSA

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