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Standardized Cryopreservation of Stem Cells

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Stem Cell Technologies in Neuroscience

Part of the book series: Neuromethods ((NM,volume 126))

Abstract

Successful commercial and clinical application of stem cells requires robust and practical cryopreservation protocols. Stem cells, particularly human embryonic stem cells and induced pluripotent stem cells, are notoriously sensitive to cryopreservation, requiring specialized protocols to maintain optimal cell viability and recovery. This chapter reviews the current state of stem cell cryopreservation and provides a clinically relevant, optimized method for controlled rate freezing and thawing of human stem cells that is reliable, inexpensive, and user friendly. This method successfully prepares stem cells for long-term cryogenic storage while ensuring maximal post-thaw cell viability.

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Author information

Authors and Affiliations

  1. MedCision, Inc., 101 Glacier Point Road, Suite E, San Rafael, CA, 94901, USA

    Maria L. Thompson Ph.D., Eric J. Kunkel & Rolf O. Ehrhardt

Authors
  1. Maria L. Thompson Ph.D.
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  2. Eric J. Kunkel
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  3. Rolf O. Ehrhardt
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Corresponding author

Correspondence to Maria L. Thompson Ph.D. .

Editor information

Editors and Affiliations

  1. Department of Pediatric Surgery Children’s Program in Regenerative Medicine, University of Texas Health Science Center at Houston McGovern Medical School, Houston, Texas, USA

    Amit K. Srivastava

  2. Center for Stem Cells and Regenerative Medicine, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, California, USA

    Evan Y. Snyder

  3. Departments of PM&R and Neurosurgery Harvard Medical School, Spaulding Rehabilitation Network and Brigham & Women’s Hospital, Boston, Massachusetts, USA

    Yang D. Teng

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Thompson, M.L., Kunkel, E.J., Ehrhardt, R.O. (2017). Standardized Cryopreservation of Stem Cells. In: Srivastava, A., Snyder, E., Teng, Y. (eds) Stem Cell Technologies in Neuroscience. Neuromethods, vol 126. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7024-7_13

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  • DOI: https://doi.org/10.1007/978-1-4939-7024-7_13

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7022-3

  • Online ISBN: 978-1-4939-7024-7

  • eBook Packages: Springer Protocols

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