Abstract
Despite the advantages of three-dimensional (3D) hydrogels for cell culture over traditional 2D plates, their clinical application is limited by inability to recapitulate the micro-architecture of complex tissues. Micropatterning can be employed to modify the homogenous micro-architecture of hydrogels. Three techniques for cell encapsulation in 3D micropatterned gels are described. The photomask and micromold techniques are used for cell encapsulation in relatively shallow patterns like disks or short rectangles but due to the presence of PDMS mold, the resolution of micromold technique is potentially higher than the photomask. The microneedle technique is often used for cell encapsulation in relatively deep microchannels within any geometry.
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Acknowledgments
This work was supported by research grants to E. Jabbari from the National Science Foundation under Award Number CBET-1403545 and the National Institute of Arthritis and Musculoskeletal and Skin Diseases of the National Institutes of Health under Award Number AR063745. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
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Moeinzadeh, S., Jabbari, E. (2017). 3D Cell Culture in Micropatterned Hydrogels Prepared by Photomask, Microneedle, or Soft Lithography Techniques. In: Koledova, Z. (eds) 3D Cell Culture. Methods in Molecular Biology, vol 1612. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7021-6_18
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DOI: https://doi.org/10.1007/978-1-4939-7021-6_18
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