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The Generation of Doubled Haploid Lines for QTL Mapping

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Plant Genomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1610))

Abstract

Recombinant inbred lines (RILs) are an essential tool for quantitative trait locus (QTL) mapping in Arabidopsis thaliana. Conventionally, the development of these lines is a time-consuming and tedious process requiring six to eight generations of selfing. Here, we describe an alternative approach: the rapid generation of RILs in A. thaliana via the creation of doubled haploids. In this method, F1 plants are crossed to an engineered haploid inducer to produce haploid plants. The chromosomes of these haploids then spontaneously double, generating immortalized homozygous F2 lines called doubled haploid RILs (DH RILs). Finally, DH RILs are genotyped using low-coverage whole-genome sequencing and are ready to be used for QTL mapping.

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Acknowledgments

We thank Anne Britt for helpful discussions. The DH RIL project in the Maloof Lab was funded by NSF grants IOS-0923752 and IOS-0820854. RM acknowledges Ramalingaswami Fellowship awarded by the Department of Biotechnology (DBT), Government of India.

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Authors and Affiliations

  1. Gregor Mendel Institute (GMI), Austrian Academy of Sciences, Vienna Biocenter (VBC), Dr. Bohr-Gasse 3, 1030, Vienna, Austria

    Daniele L. Filiault

  2. Department of Ecology and Evolutionary Biology, University of California, Irvine, Irvine, CA, USA

    Danelle K. Seymour

  3. School of Biology, Indian Institute of Science Education and Research (IISER), Thiruvananthapuram, Kerala, India

    Ravi Maruthachalam

  4. Department of Plant Biology, University of California, Davis, 1 Shields Ave., Davis, CA, USA

    Julin N. Maloof

Authors
  1. Daniele L. Filiault
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  2. Danelle K. Seymour
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  3. Ravi Maruthachalam
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  4. Julin N. Maloof
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Corresponding author

Correspondence to Julin N. Maloof .

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Editors and Affiliations

  1. Gregor Mendel Institute (GMI), Austrian Academy of Sciences, Vienna Biocenter (VBC), Vienna, Austria

    Wolfgang Busch

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Filiault, D.L., Seymour, D.K., Maruthachalam, R., Maloof, J.N. (2017). The Generation of Doubled Haploid Lines for QTL Mapping. In: Busch, W. (eds) Plant Genomics. Methods in Molecular Biology, vol 1610. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7003-2_4

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  • DOI: https://doi.org/10.1007/978-1-4939-7003-2_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7001-8

  • Online ISBN: 978-1-4939-7003-2

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