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Protein Crystallography pp 273–294Cite as

Time-Resolved Macromolecular Crystallography at Modern X-Ray Sources

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1607))

Abstract

Time-resolved macromolecular crystallography unifies protein structure determination with chemical kinetics. With the advent of fourth generation X-ray sources the time-resolution can be on the order of 10–40 fs, which opens the ultrafast time scale to structure determination. Fundamental motions and transitions associated with chemical reactions in proteins can now be observed. Moreover, new experimental approaches at synchrotrons allow for the straightforward investigation of all kind of reactions in biological macromolecules. Here, recent developments in the field are reviewed.

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Acknowledgment

M.S. thanks Vukica Šrajer for reading, and commenting on, an earlier version of the manuscript. This work is supported by the BioXFEL Science and Technology Center (NSF grant 1231306).

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Authors and Affiliations

  1. Kenwood Interdisciplinary Research Complex, Physics Department, University of Wisconsin-Milwaukee, Room 3087, 3135 North Maryland Avenue, Milwaukee, WI, 53211, USA

    Marius Schmidt

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  1. Marius Schmidt
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Correspondence to Marius Schmidt .

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Editors and Affiliations

  1. Macromolecular Crystallography Laboratory, National Cancer Institute, Frederick, Maryland, USA

    Alexander Wlodawer

  2. Synchrotron Radiation Research Section, MCL, National Cancer Institute, Argonne National Laboratory, Argonne, Illinois, USA

    Zbigniew Dauter

  3. Department of Crystallography, Faculty of Chemistry, A. Mickiewicz University, Poznan, Poland

    Mariusz Jaskolski

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Schmidt, M. (2017). Time-Resolved Macromolecular Crystallography at Modern X-Ray Sources. In: Wlodawer, A., Dauter, Z., Jaskolski, M. (eds) Protein Crystallography. Methods in Molecular Biology, vol 1607. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7000-1_11

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  • DOI: https://doi.org/10.1007/978-1-4939-7000-1_11

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