Abstract
The study of lipidomics has been dramatically enhanced by using mass spectrometry techniques.
With the purpose of identification and/or quantification of the lipids object of study, mass spectrometry has been proven to be the best approach, where three main techniques are currently being used: electrospray ionization (ESI), liquid chromatography (LC/MS), and matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (Han et al. Mass Spectrom Rev 31:134–178, 2012). In order to avoid the problems generated by gradient concentration using liquid chromatography, here we describe the method used for the lipidomics analysis using moderate and high-resolution mass spectrometry for class-specific lipid identification and quantification for phospholipid species using ESI-MS/MS.
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References
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del Carmen Piqueras, M. (2017). Utility of Moderate and High-Resolution Mass Spectrometry for Class-Specific Lipid Identification and Quantification. In: Bhattacharya, S. (eds) Lipidomics. Methods in Molecular Biology, vol 1609. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6996-8_9
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DOI: https://doi.org/10.1007/978-1-4939-6996-8_9
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-6996-8
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