Abstract
The role of sphingolipids, mainly sphingosine 1-phosphate (S1P) and the receptors for which it serves as a ligand, is an interesting and promising area in both sphingolipid and vascular biology. S1P is crucial for establishing blood flow competent blood vessels (Jung et al. Dev Cell 23(3):600–610, 2012). The role of S1P in neovascular pathology is of great interest and promising as a target for treatment. Here we describe an easy and affordable in vivo model of neovascularization by an alkali chemical burn to the cornea. This gives a consistent and easy way to quantitate methods for neovascularization.
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Acknowledgements
NAM: NIH grants EY022071, EY025256, EY021725. Foundation Fighting Blindness and Research to Prevent Blindness, USA.
JLW: T32EY023202
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Wilkerson, J.L., Mandal, N.A. (2017). Angiogenesis Model of Cornea to Understand the Role of Sphingosine 1-Phosphate. In: Bhattacharya, S. (eds) Lipidomics. Methods in Molecular Biology, vol 1609. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6996-8_23
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DOI: https://doi.org/10.1007/978-1-4939-6996-8_23
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