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Sonication-Based Basic Protocol for Liposome Synthesis

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Lipidomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1609))

Abstract

Liposomes are spherical vesicles with a wide range of sizes from nano- to micrometer scale. For the past 7–8 decades, these vesicles have occupied the interest of a variety of scientists due to its physical, chemical, and mathematical properties and, to say the least, for its immense utility and potential as delivery vehicles for toxic and nontoxic excipients into biological tissues. Methods related to selection of reagents for creation of specific liposomes of certain properties are beyond the scope of this chapter, but here, we would outline a simplistic protocol to prepare and qualify an uniform batch of simple liposome with basic cargo. This chapter will attempt to provide the reader with a starting point for this immensely potent tool to build upon the right kind of liposome, appropriate for their studies.

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Author information

Authors and Affiliations

  1. Division of Surgical Oncology, Department of Surgery, Sylvester Comprehensive Cancer Center, Miller School of Medicine, University of Miami, 1550 NW 10th Ave., Pap 303, Miami, FL, 33136, USA

    Roberto Mendez & Santanu Banerjee

Authors
  1. Roberto Mendez
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  2. Santanu Banerjee
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Corresponding author

Correspondence to Santanu Banerjee .

Editor information

Editors and Affiliations

  1. University of Miami, Miami, Florida, USA

    Sanjoy K. Bhattacharya

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Mendez, R., Banerjee, S. (2017). Sonication-Based Basic Protocol for Liposome Synthesis. In: Bhattacharya, S. (eds) Lipidomics. Methods in Molecular Biology, vol 1609. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6996-8_21

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  • DOI: https://doi.org/10.1007/978-1-4939-6996-8_21

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6995-1

  • Online ISBN: 978-1-4939-6996-8

  • eBook Packages: Springer Protocols

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