Abstract
Understanding the holistic picture of lipid homeostasis not only involves the analysis of synthesis and breakdown of lipids but also requires a thorough understanding of their transport. The transport of lipid monomers in an aqueous environment is facilitated by different lipid transfer proteins. Their universal feature is the shielding or encapsulation of the hydrophobic part of the lipid, consequently overcoming the poor solubility of lipids in water. Here we describe a method to purify lipid transfer proteins using bacterial expression. We also present three methods to validate their transfer activity.
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Acknowledgements
This work was supported by Sigrid Jusélius Foundation, Magnus Ehrnrooth Foundation, Medicinska Understödsföreningen Liv och Hälsa, Svensk Österbottniska Samfundet.
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Kjellberg, M.A., Backman, A.P.E., Möuts, A., Mattjus, P. (2017). Purification and Validation of Lipid Transfer Proteins. In: Bhattacharya, S. (eds) Lipidomics. Methods in Molecular Biology, vol 1609. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6996-8_19
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DOI: https://doi.org/10.1007/978-1-4939-6996-8_19
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