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Global Monitoring of the Mammalian Lipidome by Quantitative Shotgun Lipidomics

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Lipidomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1609))

Abstract

The emerging field of lipidomics presents the systems biology approach to identify and quantify the full lipid repertoire of cells, tissues, and organisms. The importance of the lipidome is demonstrated by a number of biological studies on dysregulation of lipid metabolism in human diseases such as cancer, diabetes, and neurodegenerative diseases. Exploring changes and regulations in the huge networks of lipids and their metabolic pathways requires a lipidomics methodology: Advanced mass spectrometry that resolves the complexity of the lipidome. Here, we report a comprehensive protocol of quantitative shotgun lipidomics that enables identification and quantification of hundreds of molecular lipid species, covering a wide range of lipid classes, extracted from cultured mammalian cells.

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Acknowledgements

This work was supported by the Scientific Committee of the Danish Cancer Society (KBVU) (K.M) (R124-A7929-15-S2).

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Authors and Affiliations

  1. Cell Death and Metabolism Unit, Center for Autophagy, Recycling and Disease, Danish Cancer Society Research Center, Copenhagen, 2100, Denmark

    Inger Ødum Nielsen, Kenji Maeda & Mesut Bilgin

Authors
  1. Inger Ødum Nielsen
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  2. Kenji Maeda
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  3. Mesut Bilgin
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Corresponding author

Correspondence to Mesut Bilgin .

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Editors and Affiliations

  1. University of Miami, Miami, Florida, USA

    Sanjoy K. Bhattacharya

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Nielsen, I.Ø., Maeda, K., Bilgin, M. (2017). Global Monitoring of the Mammalian Lipidome by Quantitative Shotgun Lipidomics. In: Bhattacharya, S. (eds) Lipidomics. Methods in Molecular Biology, vol 1609. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6996-8_12

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  • DOI: https://doi.org/10.1007/978-1-4939-6996-8_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6995-1

  • Online ISBN: 978-1-4939-6996-8

  • eBook Packages: Springer Protocols

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