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Testing PARP Inhibitors Using a Murine Xenograft Model

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Poly(ADP-Ribose) Polymerase

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1608))

Abstract

Animal models are indispensable research tools for various drug discovery and development applications. In cancer research, the growth of tumors in vivo is necessary in order to closely modulate a potential response in humans. Ectopic (subcutaneous) xenografting of human cancer cells into immunodeficient mice has long been the standard model for preclinical evaluation of novel antitumor therapeutic agents. Here, we describe the establishment and use of subcutaneous xenograft mouse model to evaluate the antitumor activity of PARP-1 inhibitors.

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Author information

Authors and Affiliations

  1. Cancer Biology Program, Fox Chase Cancer Center, 333 Cottman Avenue, Philadelphia, PA, 19111, USA

    Peter Makhov & Vladimir M. Kolenko

  2. Department of Urology, Yamagata University Faculty of Medicine, Iida-nishi 2-2-2, Yamagata, 990-9585, Japan

    Sei Naito

Authors
  1. Peter Makhov
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  2. Sei Naito
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  3. Vladimir M. Kolenko
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Corresponding author

Correspondence to Vladimir M. Kolenko .

Editor information

Editors and Affiliations

  1. Cancer Biology Program, Fox Chase Cancer Center, Philadelphia, Pennsylvania, USA

    Alexei V. Tulin

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© 2017 Springer Science+Business Media LLC

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Makhov, P., Naito, S., Kolenko, V.M. (2017). Testing PARP Inhibitors Using a Murine Xenograft Model. In: Tulin, A. (eds) Poly(ADP-Ribose) Polymerase. Methods in Molecular Biology, vol 1608. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6993-7_20

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  • DOI: https://doi.org/10.1007/978-1-4939-6993-7_20

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6992-0

  • Online ISBN: 978-1-4939-6993-7

  • eBook Packages: Springer Protocols

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