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Use of Chemicals to Inhibit DNA Replication, Transcription, and Protein Synthesis to Study Zygotic Genome Activation

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Zygotic Genome Activation

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1605))

Abstract

Maternal-to-zygotic transition is an event that developmental control of early embryos is switched from oocyte-derived factors to the zygotic genome. Ability to inhibit DNA replication, transcription, and translation is an important tool in studying events, such as zygotic genome activation, during embyogenesis. Here, we describe approaches to block DNA replication, transcription, and translation using chemical inhibitors. Then we also demonstrate how the transcript level of a maternally inherited gene, ten-eleven translocation methylcytosine dioxygenase 3, responses to the chemical treatments.

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Authors and Affiliations

  1. Department of Animal and Poultry Sciences, Virginia Tech, Blacksburg, VA, USA

    Kyungjun Uh & Kiho Lee

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  1. Kyungjun Uh
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  2. Kiho Lee
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Correspondence to Kiho Lee .

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  1. Department of Animal and Poultry Sciences, Virginia Tech, Blacksburg, Virginia, USA

    Kiho Lee

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Uh, K., Lee, K. (2017). Use of Chemicals to Inhibit DNA Replication, Transcription, and Protein Synthesis to Study Zygotic Genome Activation. In: Lee, K. (eds) Zygotic Genome Activation. Methods in Molecular Biology, vol 1605. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6988-3_13

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  • DOI: https://doi.org/10.1007/978-1-4939-6988-3_13

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6986-9

  • Online ISBN: 978-1-4939-6988-3

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