Abstract
Host cell protein content during bioprocessing of biotherapeutic proteins generated from cultured Chinese hamster ovary (CHO) cells is typically measured using immunological and gel-based methods. Estimation of HCP concentration is usually undertaken using Enzyme-Linked ImmunoSorbent Assays (ELISA), while estimation of HCP clearance/presence can be achieved by comparing 2D-PAGE images of samples and by undertaking western blotting of 2D-PAGE analyzed samples. Here, we describe the analyses of HCP content using these methodologies.
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Hogwood, C.E.M., Chiverton, L.M., Mark Smales, C. (2017). Characterization of Host Cell Proteins (HCPs) in CHO Cell Bioprocesses. In: Meleady, P. (eds) Heterologous Protein Production in CHO Cells. Methods in Molecular Biology, vol 1603. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6972-2_16
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DOI: https://doi.org/10.1007/978-1-4939-6972-2_16
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-6972-2
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