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BAC-Based Recovery of Recombinant Respiratory Syncytial Virus (RSV)

  • Christopher C. Stobart
  • Anne L. Hotard
  • Jia Meng
  • Martin L. MooreEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1602)

Abstract

Respiratory syncytial virus (RSV) is an enveloped, nonsegmented negative-strand RNA virus, which causes lower respiratory tract infections and is a leading cause of mortality in young infants. There is no available RSV vaccine and currently administered prophylactic antibodies are limited to high-risk populations. Current efforts to develop vaccines include development of live-attenuated RSV candidates. We describe here methods for preparation and recovery of recombinant RSV using an efficient bacterial artificial chromosome (BAC)-based system, expansion and plaque purification of recovered virus, and generation of master and working stocks.

Key words

Respiratory syncytial virus RSV Reverse genetics Bacterial artificial chromosome BAC Virus recovery 

Notes

Acknowledgments

This work was supported by NIH grants R01AI087798 (M.L.M.), U19 AI095227 (M.L.M.), and T32 AI074492 (C.C.S.) and the Emory Children’s Center for Childhood Infections and Vaccines (CCIV).

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Christopher C. Stobart
    • 1
    • 2
  • Anne L. Hotard
    • 1
    • 2
  • Jia Meng
    • 1
    • 2
  • Martin L. Moore
    • 1
    • 2
    Email author
  1. 1.Department of PediatricsEmory University School of MedicineAtlantaUSA
  2. 2.Children’s Healthcare of AtlantaAtlantaUSA

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