Reverse Genetics of Zika Virus

  • Chao Shan
  • Xuping Xie
  • Pei-Yong ShiEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1602)


We have established a reverse genetic system for Zika virus (ZIKV). Five shuttle plasmids were constructed and assembled into the full-length cDNA clone of ZIKV genome. To ensure the stability of the cDNA clone, we used a low copy vector (pACYC177) and a set of unique restriction enzyme sites on the ZIKV genome to assemble the full-length cDNA clone. A T7 promoter was engineered in front of the viral 5′ UTR for in vitro transcription. A hepatitis delta virus ribozyme (HDVr) sequence was engineered following the viral 3′ UTR for generation of the authentic 3′ end of the RNA transcript.

Key words

Flavivirus Zika Mosquito Dengue West Nile Guillain-Barré syndrome Microcephaly 


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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Departments of Biochemistry & Molecular Biology and Pharmacology & Toxicology, and Sealy Center for Structural Biology & Molecular BiophysicsUniversity of Texas Medical BranchGalvestonUSA

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