Abstract
Porcine reproductive and respiratory syndrome (PRRS) is a reemerging swine disease, and has become economically the most significant disease in pork production worldwide. The causative agent is PRRS virus (PRRSV), which is a member virus of the family Arteriviridae. The PRRSV genome is a single-stranded positive-sense RNA and is infectious. Two strategies in the PRRSV reverse genetics system have been employed for reconstitution of progeny virus: RNA transfection and DNA transfection. The PRRSV reverse genetics has broadly been used for studies including protein structure-function relationship, foreign gene expression, vaccine development, virulence determinants, and viral pathogenesis. Herein, we describe the modification of the pFL12 “RNA launch” reverse genetic system to the CMV promoter-driven pXJ41-FL13 “DNA launch” system. The generation of progeny PRRSV using pXJ41-FL13 is further elucidated.
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Acknowledgments
This project was supported by the Agriculture and Food Research Initiative (AFRI) Competitive Grant (2013-67015-21243) of USDA NIFA (National Institute of Food and Agriculture).
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Han, M., Ke, H., Du, Y., Zhang, Q., Yoo, D. (2017). Reverse Genetics for Porcine Reproductive and Respiratory Syndrome Virus. In: Perez, D. (eds) Reverse Genetics of RNA Viruses. Methods in Molecular Biology, vol 1602. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6964-7_3
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DOI: https://doi.org/10.1007/978-1-4939-6964-7_3
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