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Reverse Genetics for Porcine Reproductive and Respiratory Syndrome Virus

  • Mingyuan Han
  • Hanzhong Ke
  • Yijun Du
  • Qingzhan Zhang
  • Dongwan YooEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1602)

Abstract

Porcine reproductive and respiratory syndrome (PRRS) is a reemerging swine disease, and has become economically the most significant disease in pork production worldwide. The causative agent is PRRS virus (PRRSV), which is a member virus of the family Arteriviridae. The PRRSV genome is a single-stranded positive-sense RNA and is infectious. Two strategies in the PRRSV reverse genetics system have been employed for reconstitution of progeny virus: RNA transfection and DNA transfection. The PRRSV reverse genetics has broadly been used for studies including protein structure-function relationship, foreign gene expression, vaccine development, virulence determinants, and viral pathogenesis. Herein, we describe the modification of the pFL12 “RNA launch” reverse genetic system to the CMV promoter-driven pXJ41-FL13 “DNA launch” system. The generation of progeny PRRSV using pXJ41-FL13 is further elucidated.

Key words

Porcine reproductive and respiratory syndrome virus PRRS Infectious clones Reverse genetics Arterivirus Nidovirus 

Notes

Acknowledgments

This project was supported by the Agriculture and Food Research Initiative (AFRI) Competitive Grant (2013-67015-21243) of USDA NIFA (National Institute of Food and Agriculture).

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Mingyuan Han
    • 1
    • 2
  • Hanzhong Ke
    • 1
  • Yijun Du
    • 1
    • 3
  • Qingzhan Zhang
    • 1
  • Dongwan Yoo
    • 1
    Email author
  1. 1.Department of PathobiologyUniversity of Illinois at Urbana-ChampaignUrbanaUSA
  2. 2.Department of Pediatrics and Communicable DiseasesUniversity of Michigan Medical SchoolAnn ArborUSA
  3. 3.Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary MedicineShandong Academy of Agricultural SciencesJinanChina

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