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Development and Characterization of an Infectious cDNA Clone of Equine Arteritis Virus

  • Udeni B. R. BalasuriyaEmail author
  • Jianqiang Zhang
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1602)

Abstract

Development and characterization of several infectious cDNA clones of equine arteritis virus (EAV) have been described in the literature. Here we describe the assembly of the full-length infectious cDNA clone of the virulent Bucyrus strain (VBS; ATCC VR-796) of EAV in a plasmid vector. This system allows generation of infectious in vitro-transcribed (IVT) RNA from the linearized plasmid that can be transfected or electroporated into mammalian cells to produce infectious recombinant progeny virus. This is an efficient reverse genetics system that allows easy manipulation of EAV genomes to study molecular biology of the virus and pathogenesis of equine viral arteritis.

Key words

Equine arteritis virus EAV Equine viral arteritis EVA Arteriviruses Infectious cDNA clone Reverse genetics 

Notes

Acknowledgments

This work was supported by Agriculture and Food Research Initiative competitive grant no. 2013-68004-20360 from the United States Department of Agriculture National Institute of Food and Agriculture (USDA-NIFA).

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Maxwell H. Gluck Equine Research Center, Department of Veterinary ScienceUniversity of KentuckyLexingtonUSA
  2. 2.Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary MedicineIowa State UniversityAmesUSA

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