Reverse Genetics Systems for Filoviruses

  • Thomas HoenenEmail author
  • Heinz Feldmann
Part of the Methods in Molecular Biology book series (MIMB, volume 1602)


Filoviruses are among the most pathogenic viruses known to man. Reverse genetics systems, in particular full-length clone systems, allow the generation of recombinant filoviruses, which can be used to study virus biology, but also for applied uses such as screening for countermeasures. Here we describe the generation of recombinant filoviruses from cDNA.

Key words

Ebola virus Marburg virus Filoviruses Reverse genetics Full-length clone system Life-cycle modeling system Infectious clone Minigenome trVLP system 



The authors thank Allison Groseth for critical reading of the manuscript. This work was supported by the Intramural Research Program of the National Institutes of Health, NIAID.


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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Laboratory of Virology, Division of Intramural ResearchNational Institute of Allergy and Infectious Diseases, National Institutes of HealthHamiltonUSA
  2. 2.Institute of Molecular Virology and Cell BiologyFriedrich-Loeffler-InstitutGreifswaldGermany

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